Back to Search Start Over

Isolation of stem cell populations with trophic and immunoregulatory functions from human intestinal tissues: potential for cell therapy in inflammatory bowel disease

Authors :
Gianandrea Pasquinelli
Pier Luigi Tazzari
Francesco Alviano
Andrea Belluzzi
Gian Paolo Bagnara
Olavio R. Baricordi
Giacomo Lanzoni
Cosetta Marchionni
Enrico Roda
Laura Foroni
Laura Bonsi
Pasqualepaolo Pagliaro
Giulia Roda
Roberta Rizzo
Francesca Ricci
Alessandra Caponi
Roberta Costa
Francesco Lanza
Lanzoni G.
Alviano F.
Marchionni C.
Bonsi L.
Costa R.
Foroni L.
Roda G.
Belluzzi A.
Caponi A.
Ricci F.
Tazzari P.L.
Pagliaro P.
Rizzo R.
Lanza F.
Baricordi O.R.
Pasquinelli G.
Roda E.
Bagnara G.P.
Source :
Cytotherapy. 11:1020-1031
Publication Year :
2009
Publisher :
Elsevier BV, 2009.

Abstract

Bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stromal cells (MSC) are currently under evaluation in phase III clinical trials for inflammatory bowel disease and other intestinal disease manifestations. The therapeutic efficacy of these treatments may derive from a combination of the differentiation, trophic and immunomodulatory abilities of the transplanted cells. We investigated intestinal tissues as sources of MSC: such cells may support tissue-specific functions and hold advantages for engraftment and contribution in the gastrointestinal environment.Intestinal specimens were collected, and the mucosa and submucosa mechanically separated and enzymatically digested. Mesenchymal stromal populations were isolated, expanded and characterized under conditions commonly used for MSC. The differentiation potential, trophic effect and immunomodulatory ability were investigated. Results We successfully isolated and extensively expanded populations showing the typical MSC profile: CD29+, CD44+, CD73+, CD105+ and CD166+, and CD14(-), CD34(-) and CD45(-). Intestinal mucosal (IM) MSC were also CD117+, while submucosal cultures (ISM MSC) showed CD34+ subsets. The cells differentiated toward osteogenic, adipogenic and angiogenic commitments. Intestinal-derived MSC were able to induce differentiation and organization of intestinal epithelial cells (Caco-2) in three-dimensional collagen cultures. Immunomodulatory activity was evidenced in co-cultures with normal heterologous phytohemagglutinin-stimulated peripheral blood mononuclear cells. Conclusions Multipotent MSC can be isolated from intestinal mucosal and submucosal tissues. IM MSC and ISM MSC are able to perform trophic and immunomodulatory functions. These findings could open a pathway for novel approaches to intestinal disease treatment.

Details

ISSN :
14653249
Volume :
11
Database :
OpenAIRE
Journal :
Cytotherapy
Accession number :
edsair.doi.dedup.....ea92cb5e723548a4db5537b5f9ce470f