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UBP12 and UBP13 negatively regulate the activity of the ubiquitin-dependent peptidases DA1, DAR1 and DAR2
- Source :
- eLife, eLife, Vol 9 (2020)
- Publication Year :
- 2020
- Publisher :
- ELIFE SCIENCES PUBLICATIONS LTD, 2020.
-
Abstract
- Protein ubiquitination is a very diverse post-translational modification leading to protein degradation or delocalization, or altering protein activity. In Arabidopsis thaliana, two E3 ligases, BIG BROTHER (BB) and DA2, activate the latent peptidases DA1, DAR1 and DAR2 by mono-ubiquitination at multiple sites. Subsequently, these activated peptidases destabilize various positive growth regulators. Here, we show that two ubiquitin-specific proteases, UBP12 and UBP13, deubiquitinate DA1, DAR1 and DAR2, hence reducing their peptidase activity. Overexpression of UBP12 or UBP13 strongly decreased leaf size and cell area, and resulted in lower ploidy levels. Mutants in which UBP12 and UBP13 were downregulated produced smaller leaves that contained fewer and smaller cells. Remarkably, neither UBP12 nor UBP13 were found to be cleavage substrates of the activated DA1. Our results therefore suggest that UBP12 and UBP13 work upstream of DA1, DAR1 and DAR2 to restrict their protease activity and hence fine-tune plant growth and development.
- Subjects :
- 0106 biological sciences
0301 basic medicine
medicine.medical_treatment
enzymatic activity
Arabidopsis
Plant Biology
01 natural sciences
endoreduplication
Ubiquitin
Gene Expression Regulation, Plant
Biology (General)
deubiquitination
biology
Chemistry
General Neuroscience
General Medicine
Plants, Genetically Modified
Cell biology
leaf growth
Medicine
Ubiquitin-Specific Proteases
Research Article
Proteases
QH301-705.5
Science
Plant Development
Genetics and Molecular Biology
Protein degradation
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Endopeptidases
medicine
Endoreduplication
Protease
proteostasis
General Immunology and Microbiology
Cell growth
Arabidopsis Proteins
Biology and Life Sciences
Protein ubiquitination
Plant Leaves
030104 developmental biology
Proteostasis
cell proliferation
A. thaliana
General Biochemistry
biology.protein
010606 plant biology & botany
Peptide Hydrolases
Subjects
Details
- Language :
- English
- ISSN :
- 2050084X
- Database :
- OpenAIRE
- Journal :
- eLife, eLife, Vol 9 (2020)
- Accession number :
- edsair.doi.dedup.....ea7ff97c74b379f881eae981e1e62d52