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Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass
- Source :
- Communications Biology, Communications Biology, Vol 2, Iss 1, Pp 1-11 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Knockdown (KD) expression of GAlactUronosylTransferase 4 (GAUT4) in switchgrass improves sugar yield and ethanol production from the biomass. The reduced recalcitrance of GAUT4-KD transgenic biomass is associated with reduced cell wall pectic homogalacturonan and rhamnogalacturonan II content and cross-linking, and the associated increases in accessibility of cellulose to enzymatic deconstruction. To further probe the molecular basis for the reduced recalcitrance of GAUT4-KD biomass, potential recalcitrance-related factors including the physicochemical properties of lignin and hemicellulose are investigated. We show that the transgenic switchgrass have a lower abundance of ferulate and lignin-carbohydrate complex cross-linkages, reduced amounts of residual arabinan and xylan in lignin-enriched fractions after enzymatic hydrolysis, and greater coalescence and migration of lignin after hydrothermal pretreatment in comparison to the wild-type switchgrass control. The results reveal the roles of both decreased lignin-polymer and pectin cross-links in the reduction of recalcitrance in PvGAUT4-KD switchgrass.
- Subjects :
- Magnetic Resonance Spectroscopy
food.ingredient
Coumaric Acids
Pectin
Medicine (miscellaneous)
macromolecular substances
Panicum
Lignin
complex mixtures
Article
General Biochemistry, Genetics and Molecular Biology
Cell wall
chemistry.chemical_compound
food
Polysaccharides
Enzymatic hydrolysis
Ethanol fuel
Hemicellulose
Food science
Glucuronosyltransferase
Cellulose
lcsh:QH301-705.5
technology, industry, and agriculture
food and beverages
Carbohydrate
lcsh:Biology (General)
chemistry
Carbohydrate Metabolism
Pectins
General Agricultural and Biological Sciences
Subjects
Details
- ISSN :
- 23993642
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Communications Biology
- Accession number :
- edsair.doi.dedup.....ea6fb8cda7bdc1763837c169a2704284