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Analyses of propagation processes of Staphylococcus aureus bacteriophages S13′ and S25-3 in two different taxonomies by definitive screening design
- Source :
- Virus Research. 298:198406
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- To introduce phage therapy against multidrug-resistant Staphylococcus aureus in Western medicine, the establishment of phage manufacturing, particularly phage propagation, is indispensable. For the propagation of S. aureus phages, knowledge of the effects of phage types, process parameters, and analytical methodologies should be investigated. In this study, S. aureus phage propagations were studied in a flask with a new class of design of experiments, definitive screening design, using S. aureus phages S13' and S25-3 in different taxonomies. Four process parameters, namely, multiplicity of infection, bacterial density at infection, time of harvest, and temperature, were evaluated with the regression models based on the phage concentration data measured using plaque assay and quantitative polymerase chain reaction. As a result, phage propagations measured using plaque assay and quantitative polymerase chain reaction were overall similar to each other in the case of phage S13', while they differed in the case of phage S25-3. These results suggest that the propagation processes need to be developed according to phage type, and the choice of methodologies for phage concentration measurements should be carefully considered.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Cancer Research
Phage therapy
viruses
medicine.medical_treatment
Biology
medicine.disease_cause
Microbiology
03 medical and health sciences
Multiplicity of infection
Virology
Screening design
medicine
Bacteriophages
030304 developmental biology
Virus quantification
0303 health sciences
030306 microbiology
Staphylococcal Infections
Infectious Diseases
Real-time polymerase chain reaction
Phage types
Staphylococcus Phages
Western medicine
Subjects
Details
- ISSN :
- 01681702
- Volume :
- 298
- Database :
- OpenAIRE
- Journal :
- Virus Research
- Accession number :
- edsair.doi.dedup.....ea67cbb17fb138bf509b859892fa3a73