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Cell dynamics in the correct control of bone metabolism using natural treatments
- Source :
- Scopus-Elsevier
- Publication Year :
- 2006
-
Abstract
- The present study was undertaken in order to assess the efficacy of a commercial product containing calcium and silicon (Osteosil-Calcium®) on cell metabolism. MG-63 osteblast-like cells were cultured in the presence of three different drug concentrations (10, 5 and 2.5 μg/mL). Either serum-free culture and standard culture with serum were investigated. Morpho-functional tests (MTT and ALP), scanning electron microscopy (SEM), microanalysis (EDAX) and time-lapse video microscopy were performed. Cell actin cytoskeletal modification with fluorescence phalloidin staining was also tested. Our data show the in vitro functional efficacy of Osteosil-Calcium® on MG63 cell viability and ALP production. This study demonstrates its positive effect on the metabolism of the single cell and suggests wider uses of this drug in health protection and or in Regenerative Medicine therapies which are currently applied to the elderly
- Subjects :
- Silicon
Time Factors
Phalloidin
Cell Survival
Cell
030232 urology & nephrology
Biomedical Engineering
Medicine (miscellaneous)
chemistry.chemical_element
Bioengineering
Video microscopy
030204 cardiovascular system & hematology
Biology
Calcium
In Vitro Techniques
Cell Line
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Cytoskeleton
Cells, Cultured
Cell Proliferation
Analysis of Variance
Microscopy, Video
Osteoblasts
General Medicine
In vitro
Staining
medicine.anatomical_structure
chemistry
Biochemistry
Microscopy, Fluorescence
Microscopy, Electron, Scanning
Alkaline phosphatase
Subjects
Details
- ISSN :
- 03913988
- Volume :
- 28
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- The International journal of artificial organs
- Accession number :
- edsair.doi.dedup.....ea62e15d9e14b6333f38b3e43b2606c2