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Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry
- Source :
- Genetics Selection Evolution, Vol 35, Iss Suppl 1, Pp S99-S111 (2003), Genetics, Selection, Evolution : GSE
- Publication Year :
- 2003
- Publisher :
- BMC, 2003.
-
Abstract
- Several candidate genes were selected, based on their critical roles in the host's response to intracellular bacteria, to study the genetic control of the chicken response to Salmonella enteritidis (SE). The candidate genes were: inducible nitric oxide synthase (INOS), tumor necrosis factor related apoptosis inducing ligand (TRAIL), transforming growth factor β2 (TGF-β2), transforming growth factor β3 (TGF-β3), and immunoglobulin G light chain (IgL). Responses to pathogenic SE colonization or to SE vaccination were measured in the Iowa Salmonella response resource population (ISRRP). Outbred broiler sires and three diverse, highly inbred dam lines produced 508 F1 progeny, which were evaluated as young chicks for either bacterial load isolated from spleen or cecum contents after pathogenic SE inoculation, or the circulating antibody level after SE vaccination. Fragments of each gene were sequenced from the founder lines of the resource population to identify genomic sequence variation. Single nucleotide polymorphisms (SNP) were identified, then PCR-RFLP techniques were developed to genotype the F1 resource population. Linear mixed models were used for statistical analyses. Because the inbred dam lines always contributed one copy of the same allele, the heterozygous sire allele effects could be assessed in the F1 generation. Association analyses revealed significant effects of the sire allele of TRAIL-StyI on the spleen (P < 0.07) and cecum (P < 0.0002) SE bacterial load. Significant effects (P < 0.04) were found on the cecum bacterial load for TGF-β3-BsrI. Varied and moderate association was found for SE vaccine antibody response for all genes. This is the first reported study on the association of SNP in INOS, TRAIL, TGF-β2, TGF-β3, and IgL with the chicken response to SE. Identification of candidate genes to improve the immune response may be useful for marker-assisted selection to enhance disease resistance.
- Subjects :
- Candidate gene
Nitric Oxide Synthase Type II
Immunoglobulin G
TNF-Related Apoptosis-Inducing Ligand
Cecum
PCR-RFLP
Salmonella
Genotype
Genetics(clinical)
lcsh:SF1-1100
education.field_of_study
Membrane Glycoproteins
biology
poultry
General Medicine
medicine.anatomical_structure
Polymorphism, Restriction Fragment Length
lcsh:QH426-470
Population
Molecular Sequence Data
Single-nucleotide polymorphism
Polymorphism, Single Nucleotide
medicine
Genetics
Animals
Allele
education
Gene
Crosses, Genetic
Poultry Diseases
Ecology, Evolution, Behavior and Systematics
DNA Primers
Salmonella Infections, Animal
Base Sequence
Tumor Necrosis Factor-alpha
Research
candidate gene
Molecular biology
lcsh:Genetics
Salmonella enteritidis
biology.protein
Linear Models
Animal Science and Zoology
lcsh:Animal culture
Nitric Oxide Synthase
genetic
Apoptosis Regulatory Proteins
Chickens
Receptors, Transforming Growth Factor beta
Subjects
Details
- Language :
- German
- ISSN :
- 12979686
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Genetics Selection Evolution
- Accession number :
- edsair.doi.dedup.....ea521993d849c216d48f468fdd264ab1