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Vitamin D3/VDR resists diet-induced obesity by modulating UCP3 expression in muscles
- Source :
- Journal of Biomedical Science
- Publication Year :
- 2016
- Publisher :
- BioMed Central, 2016.
-
Abstract
- Background The impact of vitamin D3 (VD3) on obesity has been reported in the past. Our study was aimed at investigating the possible mechanisms by which VD3 affects obesity induced by a high fat diet. Methods Eight-week-old C57BL/6 J male mice were fed a normal- or high-fat diet for 9 weeks and were treated with a gavage of vehicle (corn oil) or cholecalciferol (50 μg/kg, daily). Body weight, white adipose tissue weight, blood lipid and glucose levels were measured. In addition, we investigated the expression of 1,25(OH)2D3 (calcitriol)/VDR-regulated genes involved in energy and lipid metabolism, such as of uncoupling protein 3 (UCP3), by using qRT-PCR in the liver, adipose tissue, skeletal muscle and C2C12, L6, and H-EMC-SS cells. We also measured UCP3 promoter transcription in the same cell lines using a Dual Luciferase Assay. Furthermore, we analyzed the binding site consensus sequences of VDR on the UCP3 promoter. Results Mice consuming a high-fat diet treated with cholecalciferol had lower body weight and adipose tissue weight and higher expression of UCP3 compared to the other treatment groups. Changes in the expression of genes correlated with calcitriol/VDR. Luciferase activity was dose-dependently associated with calcitriol/VDR levels. We confirmed the functional VDR binding site consensus sequences at -2200, -1561, -634, and +314 bp in the UCP3 promoter region. Conclusion We suggest that VD3/VDR inhibits weight gain by activating UCP3 in the muscles. Electronic supplementary material The online version of this article (doi:10.1186/s12929-016-0271-2) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Calcitriol
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Adipose tissue
Muscle Proteins
030209 endocrinology & metabolism
White adipose tissue
Biology
Calcitriol receptor
VDRE
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Internal medicine
medicine
polycyclic compounds
Animals
Uncoupling Protein 3
Pharmacology (medical)
Obesity
Muscle, Skeletal
Molecular Biology
Cholecalciferol
Biochemistry, medical
Research
Biochemistry (medical)
Promoter
Lipid metabolism
Cell Biology
General Medicine
Dietary Fats
030104 developmental biology
Endocrinology
chemistry
Gene Expression Regulation
Receptors, Calcitriol
lipids (amino acids, peptides, and proteins)
medicine.symptom
Weight gain
Transcription
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 14230127 and 10217770
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Science
- Accession number :
- edsair.doi.dedup.....ea413c68639d8c921a495ec8b25f0e87