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Inhibition of sphingosine‐1‐phosphate receptor 2 attenuated ligature‐induced periodontitis in mice
- Source :
- Oral Diseases
- Publication Year :
- 2020
- Publisher :
- John Wiley and Sons Inc., 2020.
-
Abstract
- Objectives Periodontitis is an inflammatory bone loss disease initiated by oral bacterial inflammation. Herein, we determined whether inhibition of sphingosine‐1‐phosphate receptor 2 (S1PR2, a G protein‐coupled receptor) by its specific antagonist, JTE013, could alleviate ligature‐induced periodontitis in mice. Materials and Methods C57BL/6 mice were placed with silk ligatures at the left maxillary second molar to induce experimental periodontitis. Mice were treated with JTE013 or control vehicle (dimethyl sulfoxide, DMSO) oral topically on the ligatures once daily. After 15 days of treatment, RNA was extracted from the lingual mucosal tissues to quantify IL‐1β, IL‐6, and TNF mRNA levels in the tissues. Alveolar bone loss was determined by micro‐computed tomography. Sagittal periodontal tissue sections were cut and stained by hematoxylin and eosin (H&E) for general histology, or stained by tartrate‐resistant acid phosphatase (TRAP) for osteoclasts. Results Treatment with JTE013 attenuated ligature‐induced alveolar bone loss compared with DMSO treatment. Treatment with JTE013 reduced IL‐1β, IL‐6, and TNF mRNA levels in murine gingival mucosal tissues, inhibited leukocyte infiltration in the periodontal tissues, and decreased the number of osteoclasts in the periodontal tissues compared with controls. Conclusion Oral topical administration of JTE013 alleviated periodontal inflammatory bone loss induced by ligature placement in mice.
- Subjects :
- Pathology
medicine.medical_specialty
Pyridines
medicine.medical_treatment
H&E stain
Alveolar Bone Loss
Osteoclasts
Inflammation
03 medical and health sciences
Mice
0302 clinical medicine
Osteoclast
medicine
cytokine
Animals
Ligature
bacteria
Periodontitis
General Dentistry
Sphingosine-1-Phosphate Receptors
Dental alveolus
sphingosine‐1‐phosphate receptor 2
business.industry
Periodontal Tissues
Histology
030206 dentistry
X-Ray Microtomography
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Cytokine
Otorhinolaryngology
030220 oncology & carcinogenesis
osteoclast
Pyrazoles
Original Article
medicine.symptom
business
Subjects
Details
- Language :
- English
- ISSN :
- 16010825 and 1354523X
- Volume :
- 27
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Oral Diseases
- Accession number :
- edsair.doi.dedup.....ea3b8d4e5047e9c3ca3820e48c88e2dc