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Activation of Akt and cardioprotection against reperfusion injury are maximal with only five minutes of sevoflurane postconditioning in isolated rat hearts*
- Publication Year :
- 2013
- Publisher :
- Zhejiang University Press, 2013.
-
Abstract
- It had been proved that administration of sevoflurane for the first two minutes of reperfusion effectively protects the heart against reperfusion injury in rats in vivo. Our aim was to investigate the duration of effective sevoflurane administration and its underlying mechanism in isolated rat hearts exposed to global ischemia/reperfusion (I/R) injury. Adult male Sprague-Dawley rats were randomly divided into six groups (n=12): a sham-operation group, an I/R group, and four sevoflurane postconditioning groups (S2, S5, S10, and S15). In the S2, S5, S10, and S15 groups, the duration times of sevoflurane administration were 2, 5, 10, and 15 min after the onset of reperfusion, respectively. The isolated rat hearts were mounted on the Langendorff system, and after a period of equilibrium were subjected to 40 min global ischemia and 120 min reperfusion. Left ventricular (LV) hemodynamic parameters were monitored throughout each experiment and the data at 30 min of equilibrium and 30, 60, 90, and 120 min of reperfusion were analyzed. Myocardial infarct size at the end of reperfusion (n=7 in each group) and the expression of myocardial phosphorylated Akt (p-Akt) after 15-min reperfusion were determined in a duplicate set of six groups of rat hearts (n=5 in each group). Compared with the I/R group, the S5, S10, and S15 groups had significantly improved left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP), and the maximal rate of rise or fall of the LV pressure (±dP/dt max), and decreased myocardial infarct size (P
- Subjects :
- Male
Methyl Ethers
Cardiotonic Agents
Ischemia
Hemodynamics
Myocardial Reperfusion Injury
In Vitro Techniques
General Biochemistry, Genetics and Molecular Biology
Sevoflurane
Rats, Sprague-Dawley
medicine
Animals
Myocardial infarction
General Pharmacology, Toxicology and Pharmaceutics
Cardioprotection
General Veterinary
Dose-Response Relationship, Drug
business.industry
General Medicine
medicine.disease
Rats
Enzyme Activation
Preload
Biomedicine
Treatment Outcome
Anesthesia
Platelet aggregation inhibitor
business
Reperfusion injury
Proto-Oncogene Proteins c-akt
Platelet Aggregation Inhibitors
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ea37eb2640443d167ea23bf6a64a8bbd