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Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Excessive long-term fluoride intake is associated with several health problems, including infertility. However, limited information is available on the toxic effects of fluoride exposure on the female reproductive system, especially oocyte maturation. In this study, we investigated the toxic effect of sodium fluoride (NaF) exposure on porcine oocyte maturation and its possible underlying mechanisms. Our results showed that NaF exposure during porcine oocyte maturation inhibited cumulus cell expansion and impaired polar body extrusion. Cell cycle analysis showed that NaF exposure blocked meiotic resumption, disturbed spindle dynamics, disrupted chromosome separation, and increased aneuploidy in porcine oocytes. Moreover, NaF exposure disturbed mitochondrial function, triggered DNA damage response, and induced early apoptosis in porcine oocytes. NaF exposure also induced oxidative stress, decreased GSH level, and increased cathepsin B activity in and impaired the further development potential of porcine oocytes, as indicated by a decrease in blastocyst formation rate, increase in apoptosis, and inhibition of cell proliferation. Together, these results indicate that NaF exposure impairs the maturation capacity of porcine oocytes by inhibiting cumulus cell expansion, disturbing cytoskeletal dynamics, and blocking nuclear and cytoplasmic maturation, thus decreasing the quality and affecting the subsequent embryonic development potential of porcine oocytes.
- Subjects :
- 0301 basic medicine
Swine
lcsh:Medicine
Apoptosis
medicine.disease_cause
Article
Cathepsin B
Histones
Andrology
03 medical and health sciences
Polar body
chemistry.chemical_compound
Oogenesis
0302 clinical medicine
Chromosome Segregation
Sodium fluoride
medicine
Animals
Blastocyst
lcsh:Science
Chromosome separation
Cumulus Cells
030219 obstetrics & reproductive medicine
Multidisciplinary
Cell growth
lcsh:R
Aneuploidy
Oocyte
Glutathione
Meiosis
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
chemistry
Oocytes
Sodium Fluoride
lcsh:Q
Female
Reactive Oxygen Species
Oxidative stress
DNA Damage
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ea235943b17fc9ea15e462ac7cf5e666