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A local duplication of the Melanocortin receptor 1 locus in Astyanax
- Source :
- Genome. 61(4)
- Publication Year :
- 2017
-
Abstract
- In this study, we report evidence of a novel duplication of Melanocortin receptor 1 (Mc1r) in the cavefish genome. This locus was discovered following the observation of excessive allelic diversity in a ∼820 bp fragment of Mc1r amplified via degenerate PCR from a natural population of Astyanax aeneus fish from Guerrero, Mexico. The cavefish genome reveals the presence of two closely related Mc1r open reading frames separated by a 1.46 kb intergenic region. One open reading frame corresponds to the previously reported Mc1r receptor, and the other open reading frame (duplicate copy) is 975 bp in length, encoding a receptor of 325 amino acids. Sequence similarity analyses position both copies in the syntenic region of the single Mc1r locus in 16 representative craniate genomes spanning bony fish (including Astyanax) to mammals, suggesting we discovered tandem duplicates of this important gene. The two Mc1r copies share ∼89% sequence similarity and, within Astyanax, are more similar to one another compared to other melanocortin family members. Future studies will inform the precise functional significance of the duplicated Mc1r locus and if this novel copy number variant may have adaptive significance for the Astyanax lineage.
- Subjects :
- 0301 basic medicine
Fish Proteins
Cavefish
Locus (genetics)
Biology
Genome
Article
03 medical and health sciences
Open Reading Frames
Gene Duplication
Gene duplication
Genetics
Animals
Copy-number variation
Amino Acid Sequence
Molecular Biology
Gene
Mexico
Phylogeny
Synteny
Geography
Sequence Homology, Amino Acid
Characidae
General Medicine
Open reading frame
030104 developmental biology
Receptor, Melanocortin, Type 1
Biotechnology
Subjects
Details
- ISSN :
- 14803321
- Volume :
- 61
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Genome
- Accession number :
- edsair.doi.dedup.....ea21ee10db44f4ee2f47d7550a2192db