Back to Search
Start Over
Neonatal maternal separation increases the number of p27-positive Sertoli cells in prepuberty
- Source :
- Reproductive toxicology (Elmsford, N.Y.). 102
- Publication Year :
- 2020
-
Abstract
- Neonatal maternal separation (NMS) is an experimental model for early life stress, which affects the growth and development of various organs, resulting in adverse health effects in humans and animals. In our previous study, we demonstrated that NMS [(0.5-, 1-, 2-h/day NMS, from postnatal day (PND) 1-10] induced morphological changes to the male reproductive system, including decreased Sertoli cell numbers in mouse testes at PND 70. To clarify the mechanism by which NMS decreases Sertoli cell numbers, we evaluated the effects of NMS on mouse testes at PNDs 10 and 16. At PND 10, the Sertoli cell number was not significantly different among experimental groups; however, it decreased in 0.5- and 2-h/day NMS mice at PND 16. The termination of Sertoli cell proliferation in prepuberty can be induced by p27, a cyclin-dependent kinase inhibitor. At PND 10, we observed an increase in the number of p27-positive Sertoli cells in 2-h/day NMS mice. The seminiferous tubule diameters decreased significantly in 1- and 2-h/day NMS mice, and the relative interstitial area increased in 2-h/day NMS mice. Serum corticosterone level significantly increased, and serum testosterone level significantly decreased in the 2-h/day NMS mice. At PND 16, the tubule diameters and height of seminiferous epithelium were significantly higher in 0.5- and 2-h/day NMS mice. Our results suggest that NMS disturbs serum corticosterone and testosterone levels and increases the number of p27-positive Sertoli cells at PND 10, resulting in a decrease in the number of Sertoli cells at PND 16.
- Subjects :
- Male
endocrine system
Early life stress
Sertoli cell proliferation
010501 environmental sciences
Biology
Toxicology
01 natural sciences
Andrology
03 medical and health sciences
Mice
Prepuberty
Testis
medicine
Animals
Testosterone
Spermatogenesis
030304 developmental biology
0105 earth and related environmental sciences
Cell Proliferation
0303 health sciences
Sertoli Cells
Estradiol
Kinase
Maternal Deprivation
Seminiferous Tubules
Sertoli cell
Spermatozoa
Epithelium
medicine.anatomical_structure
Seminiferous tubule
Follicle Stimulating Hormone
Subjects
Details
- ISSN :
- 18731708
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Reproductive toxicology (Elmsford, N.Y.)
- Accession number :
- edsair.doi.dedup.....ea194d7b89e20598a35a20c7ef73732b