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Viral-antigen dependence and T-cell receptor expression in leucocytes from rhabdovirus immunized trout
- Source :
- Repositorio de Resultados de Investigación del INIA, INIA: Repositorio de Resultados de Investigación del INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- This work describes the characterization of trout haematopoietic in vitro long-term cell cultures showing specific viral antigen-dependent cell (ADC) proliferation. The ADC cultures were developed from outbreed trout after surviving viral hemorrhagic septicaemia virus (VHSV) infections or after immunization with purified VHSV. For in vitro long-term proliferation of the ADC cultures, adherent (Ad) cells obtained from autologous trout were pulsed with VHSV recombinant glycoprotein G4 (G4-pulsed Ad cells) and added periodically to the cultures. ADC did not proliferate in cultures obtained from non-infected control trout treated in parallel with G4 or from VHSV survivor/VHSV immunized trout kidney donors treated with non-viral proteins. After months in culture, the ADC acquired an increasingly homogeneous morphology compatible with that of mature trout lymphocytes, secreted supernatant `factors', and were stained with rabbit antibodies to the ectodomain of recombinant trout T-cell receptor (TcR) β-chain. Together with all the above mentioned properties, the presence of TcR sequences in the ADC cultures confirmed by the expression of α- and β-chain TcR by nested PCR amplification and sequencing of the amplified bands, suggests that these ADC cultures contain trout T-like cells engaged in a VHSV response. These trout ADC cultures offer a first opportunity to further analyze fish anti-viral immunological responses.
- Subjects :
- endocrine system
animal structures
Trout
Receptors, Antigen, T-Cell, alpha-beta
animal diseases
Lymphocyte
Immunology
Kidney
Antigen-dependent cells
Virus
law.invention
Antigen
law
Leukocytes
medicine
Animals
T-cell receptor
Antigens, Viral
Cells, Cultured
General Veterinary
biology
urogenital system
T-cells
Rhabdovirus
Flow Cytometry
biology.organism_classification
Virology
Molecular biology
body regions
medicine.anatomical_structure
Cell culture
Oncorhynchus mykiss
biology.protein
Recombinant DNA
Immunization
Rabbits
Rhabdoviridae
Antibody
Cell Division
Subjects
Details
- ISSN :
- 01652427
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Veterinary Immunology and Immunopathology
- Accession number :
- edsair.doi.dedup.....ea11a37df0ef2b20e4ed26df81656eed