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Dual RNA sequencing reveals dendritic cell reprogramming in response to typhoidal Salmonella invasion

Authors :
Aulicino, A
Antanaviciute, A
Frost, J
Sousa Geros, A
Mellado, E
Attar, M
Jagielowicz, M
Hublitz, P
Sinz, J
Preciado-Llanes, L
Napolitani, G
Bowden, R
Koohy, H
Drakesmith, AH
Simmons, A
Source :
Communications Biology, Vol 5, Iss 1, Pp 1-17 (2022), Aulicino, A, Antanaviciute, A, Frost, J, Geros, A S, Mellado, E, Attar, M, Jagielowicz, M, Hublitz, P, Sinz, J, Preciado-Llanes, L, Napolitani, G, Bowden, R, Koohy, H, Drakesmith, H & Simmons, A 2022, ' Dual RNA sequencing reveals dendritic cell reprogramming in response to typhoidal Salmonella invasion ', Communications Biology, vol. 5, no. 1, 111 . https://doi.org/10.1038/s42003-022-03038-z
Publication Year :
2022
Publisher :
Springer Science and Business Media LLC, 2022.

Abstract

Salmonella enterica represent a major disease burden worldwide. S. enterica serovar Typhi (S. Typhi) is responsible for potentially life-threatening Typhoid fever affecting 10.9 million people annually. While non-typhoidal Salmonella (NTS) serovars usually trigger self-limiting diarrhoea, invasive NTS bacteraemia is a growing public health challenge. Dendritic cells (DCs) are key professional antigen presenting cells of the human immune system. The ability of pathogenic bacteria to subvert DC functions and prevent T cell recognition contributes to their survival and dissemination within the host. Here, we adapted dual RNA-sequencing to define how different Salmonella pathovariants remodel their gene expression in tandem with that of infected DCs. We find DCs harness iron handling pathways to defend against invading Salmonellas, which S. Typhi is able to circumvent by mounting a robust response to nitrosative stress. In parallel, we uncover the alternative strategies invasive NTS employ to impair DC functions.

Details

ISSN :
23993642
Volume :
5
Database :
OpenAIRE
Journal :
Communications Biology
Accession number :
edsair.doi.dedup.....ea03e4a3ab4f7521b4d67b478b0a7ae9
Full Text :
https://doi.org/10.1038/s42003-022-03038-z