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Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee
- Source :
- BMC Plant Biology 144 (11), 1-17. (2011), BMC Plant Biology, BMC Plant Biology, BioMed Central, 2011, 11 (144), pp.144. ⟨10.1186/1471-2229-11-144⟩, BMC Plant Biology, BioMed Central, 2011, 11, pp.144. ⟨10.1186/1471-2229-11-144⟩, BMC Plant Biology, Vol 11, Iss 1, p 144 (2011)
- Publication Year :
- 2011
-
Abstract
- Background Leaf rust, which is caused by the fungus Hemileia vastatrix (Pucciniales), is a devastating disease that affects coffee plants (Coffea arabica L.). Disadvantages that are associated with currently developed phytoprotection approaches have recently led to the search for alternative strategies. These include genetic manipulations that constitutively activate disease resistance signaling pathways. However, molecular actors of such pathways still remain unknown in C. arabica. In this study, we have isolated and characterized the coffee NDR1 gene, whose Arabidopsis ortholog is a well-known master regulator of the hypersensitive response that is dependent on coiled-coil type R-proteins. Results Two highly homologous cDNAs coding for putative NDR1 proteins were identified and cloned from leaves of coffee plants. One of the candidate coding sequences was then expressed in the Arabidopsis knock-out null mutant ndr1-1. Upon a challenge with a specific strain of the bacterium Pseudomonas syringae (DC3000::AvrRpt2), analysis of both macroscopic symptoms and in planta microbial growth showed that the coffee cDNA was able to restore the resistance phenotype in the mutant genetic background. Thus, the cDNA was dubbed CaNDR1a (standing for Coffea arabica Non-race specific Disease Resistance 1a). Finally, biochemical and microscopy data were obtained that strongly suggest the mechanistic conservation of the NDR1-driven function within coffee and Arabidopsis plants. Using a transient expression system, it was indeed shown that the CaNDR1a protein, like its Arabidopsis counterpart, is localized to the plasma membrane, where it is possibly tethered by means of a GPI anchor. Conclusions Our data provide molecular and genetic evidence for the identification of a novel functional NDR1 homolog in plants. As a key regulator initiating hypersensitive signalling pathways, CaNDR1 gene(s) might be target(s) of choice for manipulating the coffee innate immune system and achieving broad spectrum resistance to pathogens. Given the potential conservation of NDR1-dependent defense mechanisms between Arabidopsis and coffee plants, our work also suggests new ways to isolate the as-yet-unidentified R-gene(s) responsible for resistance to H. vastatrix.
- Subjects :
- 0106 biological sciences
Pseudomonas syringae
Coffea
Plant Science
membrane plasmique
arabica l
01 natural sciences
réaction d'hypersensibilité
Arabidopsis
lcsh:Botany
Arabidopsis thaliana
Plant Immunity
Cloning, Molecular
Disease Resistance
Plant Proteins
Genetics
0303 health sciences
Vegetal Biology
biology
plants
plasma-membrane
Plants, Genetically Modified
gène de résistance
lcsh:QK1-989
hemileia-vastatrix
arabidopsis-thaliana
hypersensitive response
signaling pathway
ndr1-1
R-proteins
coffee
defense response
leaf
plant
CaNDR1
café
plante
defense responses
Research Article
Hypersensitive response
feuille
DNA, Complementary
Molecular Sequence Data
Plant disease resistance
Genes, Plant
03 medical and health sciences
leaf rust
physiologie végétale
réaction de défense
Botany
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Amino Acid Sequence
Gene
030304 developmental biology
Plant Diseases
programmed cell-death
Hemileia vastatrix
Coffea arabica
Basidiomycota
biology.organism_classification
signaling pathways
arabidopsis
voie de signalisation
Biologie végétale
010606 plant biology & botany
Transcription Factors
rust hemileia-vastatrix
Subjects
Details
- Language :
- English
- ISSN :
- 14712229
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology 144 (11), 1-17. (2011), BMC Plant Biology, BMC Plant Biology, BioMed Central, 2011, 11 (144), pp.144. ⟨10.1186/1471-2229-11-144⟩, BMC Plant Biology, BioMed Central, 2011, 11, pp.144. ⟨10.1186/1471-2229-11-144⟩, BMC Plant Biology, Vol 11, Iss 1, p 144 (2011)
- Accession number :
- edsair.doi.dedup.....ea02cec29edee37945ac3f678eaec80c