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A genomic approach to develop a new qPCR test enabling detection of the pyricularia oryzae lineage causing wheat blast
- Source :
- Plant Disease, Plant Disease, American Phytopathological Society, 2020, 104 (1), pp.60-70. ⟨10.1094/PDIS-04-19-0685-RE⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; Rapid detection is key to managing emerging diseases because it allows their spread around the world to be monitored and limited. The first major wheat blast epidemics were reported in 1985 in the Brazilian state of Paraná. Following this outbreak, the disease quickly spread to neighboring regions and countries and, in 2016, the first report of wheat blast disease outside South America was released. This Asian outbreak was due to the trade of infected South American seed, demonstrating the importance of detection tests in order to avoid importing contaminated biological material into regions free from the pathogen. Genomic analysis has revealed that one particular lineage within the fungal species Pyricularia oryzae is associated with this disease: the Triticum lineage. A comparison of 81 Pyricularia genomes highlighted polymorphisms specific to the Triticum lineage, and this study developed a real-time PCR test targeting one of these polymorphisms. The test's performance was then evaluated in order to measure its analytical specificity, analytical sensitivity, and robustness. The C17 quantitative PCR test detected isolates belonging to the Triticum lineage with high sensitivity, down to 13 plasmid copies or 1 pg of genomic DNA per reaction tube. The blast-based approach developed here to study P. oryzae can be transposed to other emerging diseases. © 2020 The American Phytopathological Society
- Subjects :
- 0106 biological sciences
Pyricularia
Pathogen detection
Plant Science
01 natural sciences
Genome
F30 - Génétique et amélioration des plantes
Plasmid
Maladie des plantes
Pyricularia oryzae
Pathogen
Triticum
Genetics
0303 health sciences
Cereals and grains
Genome comparison
Field crops
Emerging disease
food and beverages
3. Good health
Real-time polymerase chain reaction
PCR
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infraspecific detection
Lineage (genetic)
Biology
Agent pathogène
génomique
03 medical and health sciences
030304 developmental biology
H20 - Maladies des plantes
Plante céréalière
Fungi
Outbreak
Wheat blast
biology.organism_classification
genomic DNA
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 01912917
- Database :
- OpenAIRE
- Journal :
- Plant Disease, Plant Disease, American Phytopathological Society, 2020, 104 (1), pp.60-70. ⟨10.1094/PDIS-04-19-0685-RE⟩
- Accession number :
- edsair.doi.dedup.....e9f7fd0785aafcbfc0817449dc0b7d7f