Back to Search
Start Over
Magnetically-labeled sensitized splenocytes to identify glioma by MRI: a preliminary study
- Source :
- Magnetic resonance in medicine. 58(3)
- Publication Year :
- 2007
-
Abstract
- This study investigated the feasibility of imaging the migration and incorporation of magnetically-labeled sensitized splenocytes in an experimental 9L glioma brain tumor model. Splenocytes collected from tumor-bearing (sensitized splenocytes) or control (nonsensitized splenocytes) host rats were analyzed to determine the population of different cells, labeled with ferumoxides-protamine sulfate (FePro) and injected intravenously to recipient rats (N 4, for each group) bearing intracranial 9L tumors. Day 3 postinjection of splenocytes multiecho T* 2weighted and three-dimensional (3D) gradient echo MRI were obtained using a 7 Tesla MR system. R* (1/T*) maps were created from the T*-weighted images. Signal intensities (SIs) and R* values in the tumors and contralateral brain were determined by hand drawn regions of interest (ROIs). Brain sections were stained for the evidence of administered cells. Both 3D and T* 2-weighted MRI showed low signal intensity areas in and around the tumors in rats that received labeled sensitized splenocytes. Prussian blue (PB), CD45- and CD8-positive cells were present in areas at the corresponding sites of low signal intensities seen on MRI. Rats that received labeled nonsensitized splenocytes did not show low signal intensity areas or PB positive cells in or around the implanted tumors. In conclusion, the immunogenic reaction can be exploited to delineate recurrent glioma using MRI following systemically delivered magnetically labeled sensitized splenocytes or T-cells. Magn Reson Med 58:519 –526, 2007. © 2007 Wiley-Liss, Inc.
- Subjects :
- Pathology
medicine.medical_specialty
Cell Survival
Iron
Population
Brain tumor
Contrast Media
Recurrent Glioma
CD8-Positive T-Lymphocytes
Gliosarcoma
Monocytes
Imaging, Three-Dimensional
Glioma
Cell Line, Tumor
medicine
Splenocyte
Image Processing, Computer-Assisted
Leukocytes
Animals
Radiology, Nuclear Medicine and imaging
Protamines
education
Coloring Agents
Magnetite Nanoparticles
education.field_of_study
medicine.diagnostic_test
Chemistry
business.industry
Brain Neoplasms
Brain
Magnetic resonance imaging
Dextrans
Oxides
medicine.disease
Flow Cytometry
Image Enhancement
Magnetic Resonance Imaging
Ferrosoferric Oxide
Rats, Inbred F344
Rats
9l glioma
Disease Models, Animal
Feasibility Studies
Nuclear medicine
business
Spleen
Gradient echo
Ferrocyanides
Subjects
Details
- ISSN :
- 07403194
- Volume :
- 58
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Magnetic resonance in medicine
- Accession number :
- edsair.doi.dedup.....e9d762d0dbf19106445e8aa0f64600f8