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Magnetically-labeled sensitized splenocytes to identify glioma by MRI: a preliminary study

Authors :
Ali M. Rad
Quan Jiang
Guangliang Ding
Hamid Soltanian-Zadeh
Kourosh Jafari-Khouzani
Donald J. Peck
Ali S. Arbab
Stephen L. Brown
A. S. M. Iskander
Jamie L. Churchman
Joseph A. Frank
Source :
Magnetic resonance in medicine. 58(3)
Publication Year :
2007

Abstract

This study investigated the feasibility of imaging the migration and incorporation of magnetically-labeled sensitized splenocytes in an experimental 9L glioma brain tumor model. Splenocytes collected from tumor-bearing (sensitized splenocytes) or control (nonsensitized splenocytes) host rats were analyzed to determine the population of different cells, labeled with ferumoxides-protamine sulfate (FePro) and injected intravenously to recipient rats (N 4, for each group) bearing intracranial 9L tumors. Day 3 postinjection of splenocytes multiecho T* 2weighted and three-dimensional (3D) gradient echo MRI were obtained using a 7 Tesla MR system. R* (1/T*) maps were created from the T*-weighted images. Signal intensities (SIs) and R* values in the tumors and contralateral brain were determined by hand drawn regions of interest (ROIs). Brain sections were stained for the evidence of administered cells. Both 3D and T* 2-weighted MRI showed low signal intensity areas in and around the tumors in rats that received labeled sensitized splenocytes. Prussian blue (PB), CD45- and CD8-positive cells were present in areas at the corresponding sites of low signal intensities seen on MRI. Rats that received labeled nonsensitized splenocytes did not show low signal intensity areas or PB positive cells in or around the implanted tumors. In conclusion, the immunogenic reaction can be exploited to delineate recurrent glioma using MRI following systemically delivered magnetically labeled sensitized splenocytes or T-cells. Magn Reson Med 58:519 –526, 2007. © 2007 Wiley-Liss, Inc.

Details

ISSN :
07403194
Volume :
58
Issue :
3
Database :
OpenAIRE
Journal :
Magnetic resonance in medicine
Accession number :
edsair.doi.dedup.....e9d762d0dbf19106445e8aa0f64600f8