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The Role of Csmd1 during Mammary Gland Development
- Source :
- Genes, Genes, Vol 12, Iss 162, p 162 (2021), Volume 12, Issue 2
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- The Cub Sushi Multiple Domains-1 (CSMD1) protein is a tumour suppressor which has been shown to play a role in regulating human mammary duct development in vitro. CSMD1 knockdown in vitro demonstrated increased cell proliferation, invasion and motility. However, the role of Csmd1 in vivo is poorly characterised when it comes to ductal development and is therefore an area which warrants further exploration. In this study a Csmd1 knockout (KO) mouse model was used to identify the role of Csmd1 in regulating mammary gland development during puberty. Changes in duct development and protein expression patterns were analysed by immunohistochemistry. This study identified increased ductal development during the early stages of puberty in the KO mice, characterised by increased ductal area and terminal end bud number at 6 weeks. Furthermore, increased expression of various proteins (Stat1, Fak, Akt, Slug/Snail and Progesterone receptor) was shown at 4 weeks in the KO mice, followed by lower expression levels from 6 weeks in the KO mice compared to the wild type mice. This study identifies a novel role for Csmd1 in mammary gland development, with Csmd1 KO causing significantly more rapid mammary gland development, suggesting an earlier adult mammary gland formation.
- Subjects :
- 0301 basic medicine
lcsh:QH426-470
Slug
epithelial mesenchymal transition
extracellular matrix
Organogenesis
Motility
Mammary gland formation
mammary gland development
Biology
Article
Andrology
03 medical and health sciences
Mice
0302 clinical medicine
Mammary Glands, Animal
Csmd1
Progesterone receptor
Genetics
Animals
Sexual Maturation
Protein kinase B
Genetics (clinical)
Mice, Knockout
Gene knockdown
Stat1
Fak
Cell growth
Akt
Tumor Suppressor Proteins
Membrane Proteins
biology.organism_classification
Immunohistochemistry
lcsh:Genetics
030104 developmental biology
terminal end bud
Gene Expression Regulation
030220 oncology & carcinogenesis
Female
Subjects
Details
- Language :
- English
- ISSN :
- 20734425
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....e9d3fbefcd1eb2137d533b695502885c