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Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4
- Source :
- AMB Express, AMB Express, Vol 11, Iss 1, Pp 1-9 (2021)
- Publication Year :
- 2021
-
Abstract
- Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as 213AYGAYVK219 using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.
- Subjects :
- Serotype
Monoclonal antibody
Antigenicity
medicine.drug_class
Fowl adenovirus
viruses
Biophysics
Hexon protein
Peptide
Biology
Applied Microbiology and Biotechnology
Microbiology
Epitope
medicine
chemistry.chemical_classification
Linear epitope
virus diseases
Virology
QR1-502
eye diseases
stomatognathic diseases
Capsid
chemistry
Original Article
TP248.13-248.65
Biotechnology
Subjects
Details
- ISSN :
- 21910855
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- AMB Express
- Accession number :
- edsair.doi.dedup.....e9d11f7fb8340349c85fdc67a8ec9445