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ELISA detection of semicarbazide based on a fast sample pretreatment method

Authors :
Chuanyan Ouyang
Wei Wu
Tulong Huang
Zhiyuan Fang
Zhicheng Xiang
Junhua Chen
Lingwen Zeng
Baishan Jiang
Source :
Chemical communications (Cambridge, England). 49(55)
Publication Year :
2013

Abstract

A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.

Details

ISSN :
1364548X
Volume :
49
Issue :
55
Database :
OpenAIRE
Journal :
Chemical communications (Cambridge, England)
Accession number :
edsair.doi.dedup.....e9bbf1b8f712c94ea3a6b705b955c034