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pH-Based immunoassay: explosive generation of hydrogen ions through an immuno-triggered nucleic acid exponential amplification reaction
- Source :
- The Analyst. 144(13)
- Publication Year :
- 2019
-
Abstract
- In this work, we propose a novel concept and a proof-of-concept strategy for the fabrication of a pH-based immunoassay platform with a certain degree of universality and scalability to make it adaptable for different application scenarios. The immunoreactions for the target detection are converted to pH changes through an engineered and optimized isothermal nucleic acid amplification, named exponential amplification reaction (EXPAR). Thus, a variety of well-developed methods for pH analysis, e.g. pH indicators, pH-strips and pH meters, can be applied for immunoassay directly. Here, we show that this proof-of-concept strategy is applicable for both macromolecular and micromolecular antigens by adopting human platelet-derived growth factor-BB (PDGF-BB) and chloramphenicol (CAP) as the model targets, respectively. The detection can be achieved using a colorimetric pH indicator after a 15 min reaction of the immuno-triggered isothermal nucleic acid amplification. In addition, compared with the traditional enzyme-linked immunosorbent assay (ELISA), the performance of our strategy, especially the detection limits, is improved to varying degrees for different targets, making the strategy a promising alternative for diverse application scenarios of immunoassay.
- Subjects :
- Detection limit
Chromatography
medicine.diagnostic_test
Hydrogen
Explosive material
010401 analytical chemistry
chemistry.chemical_element
02 engineering and technology
021001 nanoscience & nanotechnology
01 natural sciences
Biochemistry
0104 chemical sciences
Analytical Chemistry
Exponential function
Ion
chemistry.chemical_compound
chemistry
pH indicator
Immunoassay
Electrochemistry
medicine
Nucleic acid
Environmental Chemistry
0210 nano-technology
Spectroscopy
Subjects
Details
- ISSN :
- 13645528
- Volume :
- 144
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....e9b10a50c0e13d0a3e669fcfe47a55b5