Measuring Rates of ATP Synthesis

Here, we offer you a high-throughput assay to measure the ATP synthesis capacity in cells or isolated mitochondria. More specifically, the assay is linked to the mitochondrial' electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids). Book subtitle: Methods and Protocols ispartof: METABOLIC SIGNALING pages:97-107 ispartof: Methods in Molecular Biology vol:1862 pages:97-107 ispartof: location:United States status: published