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Reduction of renal uptake of 111In-DOTA-labeled and A700-labeled RAFT-RGD during integrin αvβ3 targeting using single photon emission computed tomography and optical imaging
- Source :
- Cancer Science, Cancer Science, Wiley Open Access, 2012, 103 (6), pp.1105-1110. ⟨10.1111/j.1349-7006.2012.02286.x⟩, Cancer Sci
- Publication Year :
- 2012
- Publisher :
- HAL CCSD, 2012.
-
Abstract
- Integrin α(v)β(3) expression is upregulated during tumor growth and invasion in newly formed endothelial cells in tumor neovasculature and in some tumor cells. A tetrameric RGD‐based peptide, regioselectively addressable functionalized template‐(cyclo‐[RGDfK])4 (RAFT‐RGD), specifically targets integrin α(v)β(3) in vitro and in vivo. When labeled with indium‐111, the RAFT‐RGD is partially reabsorbed and trapped in the kidneys, limiting its use for further internal targeted radiotherapy and imaging investigations. We studied the effect of Gelofusine on RAFT‐RGD renal retention in tumor‐bearing mice. Mice were imaged using single photon emission computed tomography and optical imaging 1 and 24 h following tracer injection. Distribution of RAFT‐RGD was further investigated by tissue removal and direct counting of the tracer. Kidney sections were analyzed by confocal microscopy. Gelofusine significantly induced a >50% reduction of the renal reabsorption of (111) In‐DOTA‐RAFT‐RGD and A700‐RAFT‐RGD, without affecting tumor uptake. Injection of Gelofusine significantly reduced the renal retention of labeled RAFT‐RGD, while increasing the tumor over healthy tissue ratio. These results will lead to the development of future therapeutic approaches. (C ancer S ci 2012; 103: 1105–1110)
- Subjects :
- Cancer Research
Pathology
030204 cardiovascular system & hematology
Kidney
Indium
Multimodal Imaging
law.invention
Mice
0302 clinical medicine
law
Tissue Distribution
ComputingMilieux_MISCELLANEOUS
biology
medicine.diagnostic_test
Chemistry
Indium Radioisotopes
General Medicine
3. Good health
medicine.anatomical_structure
Oncology
Positron emission tomography
030220 oncology & carcinogenesis
Female
medicine.medical_specialty
Metabolic Clearance Rate
Integrin
Gelofusine
Mice, Nude
Peptides, Cyclic
03 medical and health sciences
In vivo
Confocal microscopy
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Cell Line, Tumor
Organometallic Compounds
medicine
Animals
Humans
Fluorescent Dyes
Kidney metabolism
Original Articles
Integrin alphaVbeta3
HEK293 Cells
Cell culture
Positron-Emission Tomography
Polygeline
biology.protein
Biophysics
Tomography, X-Ray Computed
Subjects
Details
- Language :
- English
- ISSN :
- 13479032 and 13497006
- Database :
- OpenAIRE
- Journal :
- Cancer Science, Cancer Science, Wiley Open Access, 2012, 103 (6), pp.1105-1110. ⟨10.1111/j.1349-7006.2012.02286.x⟩, Cancer Sci
- Accession number :
- edsair.doi.dedup.....e9958981b6b75caca22561c319710b23