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18F-PEG1-Vinyl Sulfone-Labeled Red Blood Cells as Positron Emission Tomography Agent to Image Intra-Abdominal Bleeding
- Source :
- Frontiers in Medicine, Frontiers in Medicine, Vol 8 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- 18F-Labeled blood pool agents (BPAs) have attracted great attention for identifying bleeding sites. However, many BPAs are not sufficiently evaluated partially due to the limitations of labeling methods. In our previous work, we noticed that 18F-PEG1-vinyl sulfone (18F-VS) could efficiently label red blood cells (RBCs) ex vivo and in situ. However, its application as BPA is not fully evaluated. In this study, we systematically explored the feasibility of using 18F-VS-labeled RBCs as a positron emission tomography (PET) BPA for intra-abdominal bleeding diagnosis. In brief, we first optimized the labeling conditions, which lead to an 80% labeling yield of RBCs after incubating with 18F-VS in phosphate-buffered saline (PBS) at 37°C for 20 min. 18F-VS-labeled RBCs were found to be stable in vitro, which could simplify its transportation/storage for in vivo applications. In normal rat PET study, the cardiovascular system could be clearly imaged up to 5 h post injection (p.i.). An intra-abdominal hemorrhage rat model demonstrated that the 18F-VS-labeled RBCs clearly showed the dynamic changes of extravascular radioactivity due to intra-abdominal hemorrhage. Validation in the model of gastrointestinal bleeding clearly demonstrated the great potential of using 18F-VS-labeled RBCs as a BPA, which could be further evaluated in future studies.
- Subjects :
- Medicine (General)
Gastrointestinal bleeding
medicine.medical_treatment
red blood cell
Intra-Abdominal Hemorrhage
030218 nuclear medicine & medical imaging
03 medical and health sciences
R5-920
0302 clinical medicine
blood pool imaging
In vivo
medicine
positron emission tomography (PET)
Saline
Original Research
medicine.diagnostic_test
Chemistry
General Medicine
18F-vinyl sulfone
medicine.disease
In vitro
intra-abdominal hemorrhage
Red blood cell
medicine.anatomical_structure
Positron emission tomography
030220 oncology & carcinogenesis
Medicine
Ex vivo
Biomedical engineering
Subjects
Details
- ISSN :
- 2296858X
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in Medicine
- Accession number :
- edsair.doi.dedup.....e98f503894f41f13c16ad9b3a5bebca9