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Role of the A/B Region of the Human Mineralocorticoid Receptor in Aldosterone Response Selectivity
- Source :
- Biochemical and Biophysical Research Communications. 205:1610-1616
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- In transfection experiments performed with wild-type MR, the maximal transcriptional activation of a mineralocorticoid response induced by glucocorticoid was generally similar to that of aldosterone, the natural mineralocorticoid hormone. However, compared to aldosterone, glucocorticoid activity decreased when the A/B region of MR was absent. We describe in this study the synthesis and biological activities of seven mutated MRs differently truncated in the N-terminal region. Using transient expression conditions in MCF-7 cells, the N-terminal domain of MR has been shown to contain a region (residues 254-390) whose deletion led to an apparent "aldosterone selectivity". These results suggest that this region could help to maintain the most transcriptionally active conformation of MR even in the presence of ligands which are not by themselves able to fully induce such a conformation.
- Subjects :
- Transcriptional Activation
medicine.medical_specialty
Hydrocortisone
medicine.drug_class
Molecular Sequence Data
Biophysics
Biology
Transfection
Biochemistry
Dexamethasone
Cell Line
chemistry.chemical_compound
Mineralocorticoid receptor
Internal medicine
medicine
Humans
Mineralocorticoid hormone
Aldosterone
Molecular Biology
Sequence Deletion
Base Sequence
DNA
Cell Biology
Receptors, Mineralocorticoid
Endocrinology
chemistry
Mutagenesis
Mineralocorticoid
Selectivity
Glucocorticoid
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 205
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....e97db21d1a0fc591f14554938d55321c