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A Novel Fluoroimmunoassay for Detecting Ruscogenin with Monoclonal Antibodies Conjugated with CdSe/ZnS Quantum Dots
- Source :
- Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, Molecules, Vol 22, Iss 8, p 1250 (2017), Molecules; Volume 22; Issue 8; Pages: 1250
- Publication Year :
- 2017
-
Abstract
- Ruscogenin (RUS) is a steroidal sapogenin found in Ruscus aculeatus and Ophiopogon japonicus with several pharmacological activities. In the work reported herein, a novel method termed competitive fluorescence-linked immunosorbent assay (cFLISA) based on monoclonal antibodies (mAbs) coupled with quantum dots (QDs) was developed for the quick and sensitive determination of RUS in biological samples. The mAbs against RUS were conjugated with CdSe/ZnS QDs by the crossing-linking reagents and an indirect cFLISA method was developed. There was a good linear relationship between inhibition efficiency and logarithm concentration of RUS which was varied from 0.1 to 1000 ng/mL. The IC50 and limit of detection (LOD) were 9.59 ng/mL and 0.016 ng/mL respectively, which much lower than the enzyme-linked immunosorbent assay (ELISA) method. The recoveries in plasma and tissues were ranged from 82.3% to 107.0% and the intra- and inter-day precision values were below 15%. The developed cFLISA has been successfully applied to the measurement of the concentrations of RUS in biological samples of rats, and showed great potential for the tissue distribution study of RUS. The cFLISA method may provide a valuable tool for the analysis of small molecules in biological samples and such an approach could be applied to other natural products.
- Subjects :
- Ophiopogon japonicus
Fluoroimmunoassay
tissue distribution
Analytical chemistry
Pharmaceutical Science
Sapogenin
01 natural sciences
Analytical Chemistry
Rats, Sprague-Dawley
Ruscus aculeatus
Limit of Detection
Drug Discovery
Cadmium Compounds
Selenium Compounds
biology
Chemistry
Antibodies, Monoclonal
ruscogenin
Small molecule
Cross-Linking Reagents
Chemistry (miscellaneous)
Molecular Medicine
medicine.drug_class
Surface Properties
Enzyme-Linked Immunosorbent Assay
quantum dots
Conjugated system
Monoclonal antibody
fluorescent immunosorbent assay
Fluorescence
Article
lcsh:QD241-441
monoclonal antibody
lcsh:Organic chemistry
medicine
Spirostans
Animals
Humans
Physical and Theoretical Chemistry
Particle Size
IC50
Fluorescent Dyes
Detection limit
Chromatography
010405 organic chemistry
010401 analytical chemistry
Organic Chemistry
biology.organism_classification
0104 chemical sciences
Zinc Compounds
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 22
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecules (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....e97a840ae8bb09c0eecc4fd950bea1f9