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Molecular cloning and characterization of a cDNA encoding cycloartenol synthase from Fritillaria thunbergii Miq
- Source :
- African Journal of Biotechnology; Vol 11, No 26 (2012); 6896-6903
- Publication Year :
- 2012
- Publisher :
- Academic Journals, 2012.
-
Abstract
- Fritillaria thunbergii Miq., known as the bulbous plants of the genus fritillaria, produces a large amount of sterols. Homology based polymerase chain reactions (PCRs) with degenerate primers designed from the conserved sequences among the known cycloartenol synthase (CAS) resulted in cloning of a CAS from the young leaves of F. thunbergii Miq. A putative cycloartenol synthase cDNA (FtCAS) consists of a 2271 bp open reading frame, which encodes for 756 amino acids. The deduced amino acid sequence shows 82% homology to cycloartenol synthases from Dioscorea zingiberensis. Heterologous expression of the FtCAS in the methylotrophic yeast, Pichia pastoris, resulted in production of cycloartenol. Our results indicate that FtCAS encode cycloartenol synthase.Key words: Fritillaria thunbergii Miq., cycloartenol synthase, cDNA cloning, 2,3-oxidosqualene cyclase.
- Subjects :
- biology
Fritillaria
Molecular cloning
biology.organism_classification
Applied Microbiology and Biotechnology
Molecular biology
Fritillaria thunbergii
Conserved sequence
Pichia pastoris
chemistry.chemical_compound
Fritillaria thunbergii Miq., cycloartenol synthase, cDNA cloning, 2,3-oxidosqualene cyclase
Cycloartenol synthase
chemistry
Biochemistry
Complementary DNA
Genetics
biology.protein
Cycloartenol
Agronomy and Crop Science
Molecular Biology
Biotechnology
Subjects
Details
- ISSN :
- 16845315 and 68966903
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- African Journal of Biotechnology
- Accession number :
- edsair.doi.dedup.....e97201824b597d2d8614832c04f4ee75