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Gliclazide Does Not Fully Prevent 2-Deoxy-D-Ribose-Induced Oxidative Damage Because It Does Not Restore Glutathione Content in a Pancreatic β-Cell Line
- Source :
- Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2012 (2012)
- Publication Year :
- 2012
- Publisher :
- Hindawi Publishing Corporation, 2012.
-
Abstract
- We compared the effects of gliclazide, an antidiabetic agent with antioxidant properties, andN-acetyl-L-cysteine (NAC), a glutathione precursor, in protecting against 2-deoxy-D-ribose- (dRib-) induced oxidative damage in HIT-T15 cells. Using trypan blue staining and flow cytometry with annexin V/PI staining, gliclazide treatment slightly reversed dRib-induced cell death and apoptosis, and NAC treatment markedly reduced both measures. Likewise, flow cytometry using DHR 123 staining showed that the levels of dRib-induced reactive oxygen species (ROS) were partially suppressed by gliclazide and completely inhibited by NAC. Using electron spin resonance spectrometry, gliclazide and NAC scavenged hydroxyl radicals generated by Fenton reaction to a similar degree in a cell-free system. NAC, but not gliclazide, completely restored the intracellular glutathione depleted by dRib using monochlorobimane fluorescence and glutathione assays. Thus, gliclazide treatment suppressed dRib-induced oxidative damage in HIT-T15 cells less than NAC did because gliclazide did not restore the intracellular glutathione content as effectively as NAC. In addition, the elevation of intracellular glutathione rather than free radical scavenging might be an important mechanism for protecting against dRib-induced oxidative damage in aβ-cell line.
- Subjects :
- Aging
Programmed cell death
Antioxidant
Article Subject
Cell Survival
medicine.medical_treatment
Apoptosis
Pharmacology
medicine.disease_cause
Biochemistry
Cell Line
Acetylcysteine
chemistry.chemical_compound
Cricetinae
Insulin-Secreting Cells
medicine
Animals
Hypoglycemic Agents
Gliclazide
Drug Interactions
lcsh:QH573-671
chemistry.chemical_classification
Reactive oxygen species
lcsh:Cytology
Deoxyribose
Hydroxyl Radical
Cell Biology
General Medicine
Glutathione
Oxidative Stress
chemistry
Reactive Oxygen Species
Oxidative stress
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19420900
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....e92e07199c0c645fc5e7e22938067fcf
- Full Text :
- https://doi.org/10.1155/2012/390678