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No Difference in Glycosphingolipid Metabolism and Mitochondrial Function in Glucocorticoid-Induced Insulin Resistance in Healthy Men
- Source :
- Brands, M, van Raalte, D H, Ferraz, M J, Sauerwein, H P, Verhoeven, A J, Aerts, J M F G, Diamant, M & Serlie, M J 2013, ' No Difference in Glycosphingolipid Metabolism and Mitochondrial Function in Glucocorticoid-Induced Insulin Resistance in Healthy Men ', Journal of Clinical Endocrinology and Metabolism, vol. 98, no. 3, pp. 1219-1225 . https://doi.org/10.1210/jc.2012-3266, Journal of Clinical Endocrinology and Metabolism, 98(3), 1219-1225. The Endocrine Society, Journal of clinical endocrinology and metabolism, 98(3), 1219-1225. The Endocrine Society
- Publication Year :
- 2013
-
Abstract
- Objective: Glucocorticoids (GCs) are well known to induce insulin resistance; however, mechanisms that cause the impairement of the insulin signaling pathway have not yet been identified. In this study we measured whether GC-induced insulin resistance in humans is related to changes in muscle ceramide, GM3, and muscle mitochondrial function. Methods: In a randomized, placebo-controlled, double-blind, dose-response intervention study, 32 healthy males (aged 22 ± 3 years; body mass index 22.4 ± 1.7 kg/m(-2)) were allocated to prednisolone (PRED) 7.5 mg once daily (n = 12), PRED 30 mg once daily (n = 12), or placebo (n = 8) for 2 weeks using block randomization. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp before and after treatment. Muscle biopsies were performed to measure ceramide, monosialodihexosylganglioside (GM3), and mitochondrial function. Results: Peripheral insulin sensitivity was dose dependently decreased after the PRED treatment. Muscle ceramide and GM3 concentration and mitochondrial function were not altered by 2 weeks of PRED treatment. Conclusion: Short-term GC treatment dose dependently impaired whole-body insulin sensitivity in healthy males, without concomitant changes in muscle ceramide, GM3, or mitochondrial function. These findings suggest that other mechanisms play a role in GC-related impairment of insulin sensitivity
- Subjects :
- Adult
Blood Glucose
Male
medicine.medical_specialty
Ceramide
Endocrinology, Diabetes and Metabolism
Prednisolone
Clinical Biochemistry
Mitochondrion
Biology
Placebo
Ceramides
Biochemistry
Glycosphingolipids
Placebos
chemistry.chemical_compound
Young Adult
Endocrinology
Insulin resistance
Double-Blind Method
Internal medicine
medicine
G(M3) Ganglioside
Humans
Insulin
Muscle, Skeletal
Glucocorticoids
Dose-Response Relationship, Drug
Biochemistry (medical)
Glucose clamp technique
medicine.disease
Mitochondria
Dose–response relationship
chemistry
Glucose Clamp Technique
Insulin Resistance
Energy Metabolism
Glucocorticoid
medicine.drug
Signal Transduction
Subjects
Details
- ISSN :
- 0021972X
- Database :
- OpenAIRE
- Journal :
- Brands, M, van Raalte, D H, Ferraz, M J, Sauerwein, H P, Verhoeven, A J, Aerts, J M F G, Diamant, M & Serlie, M J 2013, ' No Difference in Glycosphingolipid Metabolism and Mitochondrial Function in Glucocorticoid-Induced Insulin Resistance in Healthy Men ', Journal of Clinical Endocrinology and Metabolism, vol. 98, no. 3, pp. 1219-1225 . https://doi.org/10.1210/jc.2012-3266, Journal of Clinical Endocrinology and Metabolism, 98(3), 1219-1225. The Endocrine Society, Journal of clinical endocrinology and metabolism, 98(3), 1219-1225. The Endocrine Society
- Accession number :
- edsair.doi.dedup.....e90f6b48dfdf67c1d97cec70f266960f