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Collagenous Extracellular Matrix of Cartilage Submitted to Mechanical Forces Studied by Second Harmonic Generation Microscopy
- Source :
- Photochemistry and Photobiology, Photochemistry and Photobiology, Wiley, 2010, 86 (2), pp.302-10. ⟨10.1111/j.1751-1097.2009.00648.x⟩
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- International audience; Osteoarthritis is a degenerative pathology leading to degradation of the extracellular matrix (ECM). Similar effects can be visualized when applying mechanical or biochemical constraints on cartilaginous tissue. Here, we characterized modification of the ECM appearing under mechanical compression and/or biochemical action (hypoxia environment, nitric oxide and collagenase action). In recent decades, multiphoton microscopy has proved its interest for observing living, thick and opaque biological tissues. Thus, the main components of the cartilaginous ECM can be observed without fluorescent labeling. In particular, the collagen network emits strong second harmonic generation (SHG) signal which could be collected at half of the excitation wavelength. Combining autofluorescence and SHG signal detection enables to obtain complementary structural information. Here, we proved that multiphoton microscopy represents an appropriate tool for ex vitro cartilage imaging. First, we showed that SHG signal specifically comes from collagen (collagenase digestion). Further, we verified that the use of an appropriate band-pass filter enables to reject the autofluorescence from the ECM. Once this specificity was shown, we followed modification of the cartilage ECM submitted to mechanical or biochemical constraints (compression, enzymatic digestion). By performing textural analysis of SHG images (Haralick's method), we showed the restructuration of the collagen network according to constraints.
- Subjects :
- 0206 medical engineering
Nanotechnology
02 engineering and technology
MESH: Extracellular Matrix
Biochemistry
Extracellular matrix
03 medical and health sciences
MESH: Osteoarthritis
MESH: Collagen
Osteoarthritis
Microscopy
Collagen network
Cartilaginous Tissue
medicine
Physical and Theoretical Chemistry
MESH: Biomechanics
030304 developmental biology
0303 health sciences
Chemistry
Cartilage
General Medicine
Second Harmonic Generation Microscopy
020601 biomedical engineering
Biomechanical Phenomena
Extracellular Matrix
Autofluorescence
Microscopy, Fluorescence, Multiphoton
medicine.anatomical_structure
MESH: Cartilage
MESH: Microscopy, Fluorescence, Multiphoton
Collagenase
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Collagen
Biomedical engineering
medicine.drug
Subjects
Details
- ISSN :
- 17511097 and 00318655
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Photochemistry and Photobiology
- Accession number :
- edsair.doi.dedup.....e906a28d0554115d8d9bf5c87d912780