Back to Search
Start Over
Cytokine and chemokine responses in a cerebral malaria-susceptible or -resistant strain of mice to Plasmodium berghei ANKA infection: early chemokine expression in the brain
- Source :
- International Immunology. 15:633-640
- Publication Year :
- 2003
- Publisher :
- Oxford University Press (OUP), 2003.
-
Abstract
- A comparative study was carried out on cytokine and chemokine responses in a cerebral malaria (CM)-susceptible or -resistant strain of mice (C57BL/6 or BALB/c respectively) in Plasmodium berghei ANKA infection. C57BL/6 mice died by 10 days after infection when parasitemia was approximately 15-20% with cerebral symptoms, while BALB/c mice survived until week 3 after infection. Although both strains showed T(h)1-skewed responses on day 4 after infection, significantly higher levels of IFN-gamma, tumor necrosis factor (TNF)-alpha and NO were observed during the course of the infection in BALB/c, suggesting that T(h)1 responses are involved in the resistance. Interestingly, in the brain, both strains expressed IFN-inducible protein of 10 kDa (IP-10) and monocyte chemotactic protein (MCP)-1 genes as early as at 24 h post-infection, whereas some differences were observed between both strains thereafter, i.e. enhanced expression of RANTES in C57BL/6, and of IFN-gamma and TNF-alpha in BALB/c respectively. Moreover, the expression of IP-10 and MCP-1 genes in KT-5, an astrocyte cell line, was induced in vitro upon stimulation with a crude antigen of malaria parasites. These results suggest that the direct involvement of brain parenchymal cells takes place in response to plasmodial infection, providing a new aspect to analyze possible mechanisms of CM. This is the first report on the chemokine expression in neuroglial cells in response to malaria infection.
- Subjects :
- Chemokine
Plasmodium berghei
medicine.medical_treatment
Immunology
Parasitemia
Nitric Oxide
Mice
Antigen
medicine
Animals
Immunology and Allergy
Mice, Inbred BALB C
biology
Monocyte
Brain
General Medicine
biology.organism_classification
medicine.disease
Malaria
Mice, Inbred C57BL
medicine.anatomical_structure
Cytokine
Cerebral Malaria
Astrocytes
biology.protein
Cytokines
Tumor necrosis factor alpha
Chemokines
Spleen
Subjects
Details
- ISSN :
- 14602377
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....e9007b0b91042d271b2bf7db55cad4c9