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Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108 (7), pp.2993-2998, Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (7), pp.2993-2998. ⟨10.1073/pnas.1015326108⟩, Proceedings Of The National Academy Of Sciences Of The United States Of America (0027-8424) (Natl Acad Sciences), 2011-02, Vol. 108, N. 7, P. 2993-2998, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108 (7), pp.2993-2998. ⟨10.1073/pnas.1015326108⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera , require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for β-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase Cg -EcSOD, is used as an opsonin mediating the OmpU-promoted phagocytosis through its RGD sequence. Finally, the endocytosed bacteria were found to survive intracellularly, evading the host defense by preventing acidic vacuole formation and limiting reactive oxygen species production. We conclude that ( i ) V. splendidus is a facultative intracellular pathogen that manipulates host defense mechanisms to enter and survive in host immune cells, and ( ii ) that OmpU is a major determinant of host cell invasion in Vibrio species, used by V. splendidus LGP32 to attach and invade oyster hemocytes through opsonisation by the oyster plasma Cg -EcSOD.
- Subjects :
- Oyster
Hemocytes
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Host–pathogen interaction
Green Fluorescent Proteins
invertebrate
Porins
Virulence
Human pathogen
host-pathogen interaction
Polymerase Chain Reaction
Statistics, Nonparametric
Microbiology
03 medical and health sciences
Tandem Mass Spectrometry
biology.animal
Animals
Crassostrea
oxidative burst
Adhesins, Bacterial
innate immunity
ComputingMilieux_MISCELLANEOUS
Vibrio
030304 developmental biology
[SDV.EE]Life Sciences [q-bio]/Ecology, environment
Analysis of Variance
0303 health sciences
Microscopy, Confocal
Multidisciplinary
Innate immune system
biology
Superoxide Dismutase
030306 microbiology
Intracellular parasite
mollusk
Biological Sciences
Flow Cytometry
Actin cytoskeleton
biology.organism_classification
Immunity, Innate
[SHS.ENVIR]Humanities and Social Sciences/Environmental studies
Host-Pathogen Interactions
Electrophoresis, Polyacrylamide Gel
France
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108 (7), pp.2993-2998, Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (7), pp.2993-2998. ⟨10.1073/pnas.1015326108⟩, Proceedings Of The National Academy Of Sciences Of The United States Of America (0027-8424) (Natl Acad Sciences), 2011-02, Vol. 108, N. 7, P. 2993-2998, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2011, 108 (7), pp.2993-2998. ⟨10.1073/pnas.1015326108⟩
- Accession number :
- edsair.doi.dedup.....e8f8d978babe7126dadd000f8f75ac29