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circRNA hsa_circ_0005909 Predicts Poor Prognosis and Promotes the Growth, Metastasis, and Drug Resistance of Non-Small-Cell Lung Cancer via the miRNA-338-3p/SOX4 Pathway
- Source :
- Disease Markers, Disease Markers, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Background. Circular RNAs (circRNAs) are powerful factors in regulating various cancer behaviors. It has been manifested in previous researches that circular RNA hsa_circ_0005909 (circ_0005909) exhibits a regulatory function in osteosarcoma. However, there are no other studies on whether circ_0005909 displays potential functions on the progression of non-small-cell lung cancer (NSCLC). Methods. RT-PCR was applied to examine the expression of circ_0005909 in NSCLC. To study the specific behaviors of NSCLC cells after circ_0005909 knockdown, cell counting kit-8 (CCK-8) assays, colony formation assays, Transwell assays, and xenograft tumor model assays were conducted. Bioinformatics and luciferase reporter assays were employed to study the association among circ_0005909, miRNA-338-3p, and SOX4. Results. In this research, our group firstly showed that circ_0005909 expressions were distinctly increased in NSCLC specimens and cell lines. Clinical studies revealed that high circ_0005909 expressions were associated with poor prognosis of NSCLC patients. Functionally, knockdown of circ_0005909 was observed to suppress the proliferation, metastasis, and drug resistance of NSCLC cells. In the terms of mechanism, circ_0005909 could act as a sponge of miRNA-338-3p, and miRNA-338-3p could target SOX4. In addition, miRNA-338-3p inhibitors reversed the suppressor ability of circ_0005909 silence on NSCLC behaviors. Conclusions. circ_0005909 promoted the progression of NSCLC via the modulation of the miRNA-338-3p/SOX4 axis, which may be a therapeutic target for NSCLC.
- Subjects :
- Male
Medicine (General)
Lung Neoplasms
Article Subject
Clinical Biochemistry
SOXC Transcription Factors
Metastasis
Mice
SOX4
R5-920
Cell Movement
Circular RNA
Carcinoma, Non-Small-Cell Lung
Cell Line, Tumor
microRNA
Genetics
medicine
Animals
Humans
Lung cancer
Molecular Biology
Cell Proliferation
Gene knockdown
business.industry
Biochemistry (medical)
Cancer
RNA, Circular
General Medicine
Prognosis
medicine.disease
Up-Regulation
respiratory tract diseases
Gene Expression Regulation, Neoplastic
MicroRNAs
A549 Cells
Drug Resistance, Neoplasm
Cancer research
Osteosarcoma
Female
business
Neoplasm Transplantation
Research Article
Subjects
Details
- ISSN :
- 18758630 and 02780240
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- Disease Markers
- Accession number :
- edsair.doi.dedup.....e8f60bda776200fe3fc28b827bd4661e
- Full Text :
- https://doi.org/10.1155/2021/8388512