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Inhibition of herpes simplex virus types 1 and 2 in vitro infection by sulfated derivatives of Escherichia coli K5 polysaccharide
- Publication Year :
- 2008
-
Abstract
- Herpes simplex virus type 1 (HSV-1) and HSV-2 are neurotropic viruses and common human pathogens causing major public health problems such as genital herpes, a sexually transmitted disease also correlated with increased transmission and replication of human immunodeficiency virus type 1 (HIV-1). Therefore, compounds capable of blocking HIV-1, HSV-1, and HSV-2 transmission represent candidate microbicides with a potential added value over that of molecules acting selectively against either infection. We report here that sulfated derivatives of the Escherichia coli K5 polysaccharide, structurally highly similar to heparin and previously shown to inhibit HIV-1 entry and replication in vitro, also exert suppressive activities against both HSV-1 and HSV-2 infections. In particular, the N,O-sulfated [K5-N,OS(H)] and O-sulfated epimerized [Epi-K5-OS(H)] forms inhibited the infection of Vero cells by HSV-1 and -2, with 50% inhibitory concentrations (IC 50 ) between 3 ± 0.05 and 48 ± 27 nM, and were not toxic to the cells at concentrations as high as 5 μM. These compounds impaired the early steps of HSV-1 and HSV-2 virion attachment and entry into host cells and reduced the cell-to-cell spread of HSV-2. Since K5-N,OS(H) and Epi-K5-OS(H) also inhibit HIV-1 infection, they may represent valid candidates for development as topical microbicides preventing sexual transmission of HIV-1, HSV-1, and HSV-2.
- Subjects :
- Sexually transmitted disease
Sexual transmission
HSV2
Herpesvirus 2, Human
viruses
HSV1
inhibition
sulfated derivatives of K5 E.Coli polysaccharide
Herpesvirus 1, Human
Biology
medicine.disease_cause
Antiviral Agents
Virus
Herpesviridae
Microbiology
Cell Line, Tumor
Chlorocebus aethiops
Escherichia coli
medicine
Animals
Humans
Pharmacology (medical)
Vero Cells
Bacterial Capsules
Recombination, Genetic
Pharmacology
Sulfates
Epithelial Cells
Entry into host
Virology
Microbicides for sexually transmitted diseases
Infectious Diseases
Herpes simplex virus
Vero cell
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e8e47287792047be3db159fda703ac53