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A Caenorhabditis elegans protein with a PRDM9-like SET domain localizes to chromatin-associated foci and promotes spermatocyte gene expression, sperm production and fertility
- Source :
- PLoS Genetics, Vol 14, Iss 4, p e1007295 (2018), PLoS Genetics, PLoS, PLoS Genetics, 14(4). Public Library of Science
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- To better understand the tissue-specific regulation of chromatin state in cell-fate determination and animal development, we defined the tissue-specific expression of all 36 C. elegans presumptive lysine methyltransferase (KMT) genes using single-molecule fluorescence in situ hybridization (smFISH). Most KMTs were expressed in only one or two tissues. The germline was the tissue with the broadest KMT expression. We found that the germline-expressed C. elegans protein SET-17, which has a SET domain similar to that of the PRDM9 and PRDM7 SET-domain proteins, promotes fertility by regulating gene expression in primary spermatocytes. SET-17 drives the transcription of spermatocyte-specific genes from four genomic clusters to promote spermatid development. SET-17 is concentrated in stable chromatin-associated nuclear foci at actively transcribed msp (major sperm protein) gene clusters, which we term msp locus bodies. Our results reveal the function of a PRDM9/7-family SET-domain protein in spermatocyte transcription. We propose that the spatial intranuclear organization of chromatin factors might be a conserved mechanism in tissue-specific control of transcription.<br />National Institutes of Health (U.S.) (grant GM24663)<br />NIH/National Cancer Institute Physical Sciences Oncology Center (U54CA1438 74)<br />National Institutes of Health (U.S.) Pioneer Award (8 DP1 CA174420- 05)
- Subjects :
- 0301 basic medicine
RNA, Messenger/genetics
Male
Cancer Research
Transcription, Genetic
Nematoda
Polycomb-Group Proteins
Gene Expression
Spermatocyte
Biochemistry
Histone-Lysine N-Methyltransferase/genetics
Histones
Spermatocytes/metabolism
Spermatocytes
Animal Cells
Gene expression
Caenorhabditis elegans/enzymology
Genetics (clinical)
Caenorhabditis elegans
In Situ Hybridization, Fluorescence
In Situ Hybridization
Regulation of gene expression
Chromosome Biology
Nuclear Proteins
Eukaryota
Animal Models
Spermatids
Chromatin
3. Good health
Cell biology
Major sperm protein
medicine.anatomical_structure
Experimental Organism Systems
Multigene Family
Epigenetics
Cellular Types
Transcription
Research Article
lcsh:QH426-470
DNA transcription
Biology
Research and Analysis Methods
Fluorescence
Chromatin/metabolism
03 medical and health sciences
Caenorhabditis elegans Proteins/genetics
Model Organisms
Genetic
DNA-binding proteins
Polycomb-Group Proteins/genetics
medicine
Genetics
Animals
RNA, Messenger
Caenorhabditis elegans Proteins
Molecular Biology
Gene
Ecology, Evolution, Behavior and Systematics
Spermatid
Organisms
Biology and Life Sciences
Proteins
Histone-Lysine N-Methyltransferase
Cell Biology
biology.organism_classification
Invertebrates
Sperm
Nuclear Proteins/genetics
lcsh:Genetics
030104 developmental biology
Fertility
Germ Cells
Gene Expression Regulation
Caenorhabditis
Fertility/genetics
RNA
Messenger/genetics
Subjects
Details
- Language :
- English
- ISSN :
- 15537404 and 15537390
- Volume :
- 14
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS Genetics
- Accession number :
- edsair.doi.dedup.....e8e23adbe2d2c59a328fe97305d770d0