Back to Search
Start Over
Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana
- Source :
- Proceedings of the National Academy of Sciences. 101:12142-12147
- Publication Year :
- 2004
- Publisher :
- Proceedings of the National Academy of Sciences, 2004.
-
Abstract
- Signals generated by cryptochrome (CRY) blue-light photoreceptors are responsible for a variety of developmental and circadian responses in plants. The CRYs are also identified as circadian blue-light photoreceptors in Drosophila and components of the mammalian circadian clock. These flavoproteins all have an N-terminal domain that is similar to photolyase, and most have an additional C-terminal domain of variable length. We present here the crystal structure of the photolyase-like domain of CRY-1 from Arabidopsis thaliana . The structure reveals a fold that is very similar to photolyase, with a single molecule of FAD noncovalently bound to the protein. The surface features of the protein and the dissimilarity of a surface cavity to that of photolyase account for its lack of DNA-repair activity. Previous in vitro experiments established that the photolyase-like domain of CRY-1 can bind Mg·ATP, and we observe a single molecule of an ATP analog bound in the aforementioned surface cavity, near the bound FAD cofactor. The structure has implications for the signaling mechanism of CRY blue-light photoreceptors.
- Subjects :
- Models, Molecular
endocrine system
Adenylyl Imidodiphosphate
Photosynthetic Reaction Center Complex Proteins
Static Electricity
Circadian clock
Arabidopsis
Biology
Crystallography, X-Ray
chemistry.chemical_compound
Adenosine Triphosphate
Protein structure
Cryptochrome
Arabidopsis thaliana
Photolyase
Flavin adenine dinucleotide
Binding Sites
Multidisciplinary
Flavoproteins
Arabidopsis Proteins
Biological Sciences
biology.organism_classification
Recombinant Proteins
Protein Structure, Tertiary
Cryptochromes
Biochemistry
chemistry
Flavin-Adenine Dinucleotide
Biophysics
Deoxyribodipyrimidine Photo-Lyase
Signal Transduction
Cryptochrome-1
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....e8e0ae40fd2dd2981b4debea800c9c89