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Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition
- Source :
- BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-12 (2019), BMC Complementary and Alternative Medicine
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- BackgroundVernonia anthelmintica (L.) willdis a traditional urgur herb in China for a long history. Its alcohol extract (AVE) has been proved to promote hair follicle growth in C57BL/6 mice. We conducted this study to investigate the hair-growth effects of AVE in stressed mice and its possible mechanism of action.MethodsThe hair-follicle growth effects of AVE were examined by in vivo and in vitro study. We exposed C57BL/6 male mice to chronic restraint stress to induce murine hair follicle growth inhibition. The effects of AVE were examined by histological analysis, immunofluorescence for Ki67 and cytokeratin 19 immunoreactivity, western blot assay in tyrosinase and related proteins expressions and immunofluorescence for nerve fibers. In organ culture of mouse vibrissae follicles, we used substance P as a catagen-inducing factor of hair follicle growth, and measured the elongation of hair shafts and expression of neurokinin-1 receptor protein by application of AVE.ResultsOur results showed that AVE counteract murine hair follicle growth inhibition caused by chronic restraint stress via inducing the conversion of telogen to anagen and inhibiting catagen premature, increasing bulb keratinocytes and bulge stem cells proliferation, promoting melanogenesis, and reducing the numbers of substance P and calcitonin gene-related peptide nerve fibers. Furthermore, AVE also counteracted murine hair follicle growth inhibition caused by substance P in organ culture.ConclusionThese results suggest that AVE counteract stress-induced hair follicle growth inhibition in C57BL/6 mice in vivo and in vitro, and may be an effective new candidate for treatment of stress-induced hair loss.
- Subjects :
- Male
Restraint, Physical
medicine.medical_specialty
Melanogenesis
Substance P
Nerve fibers
Organ culture
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
Mice
Chronic restraint stress
0302 clinical medicine
In vivo
Stress, Physiological
Internal medicine
medicine
Animals
Receptor
030304 developmental biology
Hair follicle
0303 health sciences
integumentary system
Chemistry
Plant Extracts
General Medicine
lcsh:Other systems of medicine
medicine.disease
lcsh:RZ201-999
Mice, Inbred C57BL
Hair loss
medicine.anatomical_structure
Endocrinology
Complementary and alternative medicine
Mechanism of action
Vernonia anthelmintica (L.) willd extract (AVE)
medicine.symptom
Growth inhibition
Vernonia
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....e8dff3e2316486f4838dedac971b5020