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Exogenous γ-aminobutyric acid (GABA) affects pollen tube growth via modulating putative Ca2+-permeable membrane channels and is coupled to negative regulation on glutamate decarboxylase
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2014
- Publisher :
- Oxford University Press, 2014.
-
Abstract
- Summary This work reveals how tobacco pistil and style may communicate with pollen tubes and regulate their growth during fertilization via the γ-aminobutyric acid–Ca2+-permeable channel–glutamate decarboxylase–calmodulin signalling pathway.<br />γ-Aminobutyric acid (GABA) is implicated in pollen tube growth, but the molecular and cellular mechanisms that it mediates are largely unknown. Here, it is shown that exogenous GABA modulates putative Ca2+-permeable channels on the plasma membranes of tobacco pollen grains and pollen tubes. Whole-cell voltage-clamp experiments and non-invasive micromeasurement technology (NMT) revealed that the influx of Ca2+ increases in pollen tubes in response to exogenous GABA. It is also demonstrated that glutamate decarboxylase (GAD), the rate-limiting enzyme of GABA biosynthesis, is involved in feedback controls of Ca2+-permeable channels to fluctuate intracellular GABA levels and thus modulate pollen tube growth. The findings suggest that GAD activity linked with Ca2+-permeable channels relays an extracellular GABA signal and integrates multiple signal pathways to modulate tobacco pollen tube growth. Thus, the data explain how GABA mediates the communication between the style and the growing pollen tubes.
- Subjects :
- 0106 biological sciences
Ca2+-permeable channel
Calmodulin
Physiology
Glutamate decarboxylase
Plant Science
Pollen Tube
medicine.disease_cause
Real-Time Polymerase Chain Reaction
01 natural sciences
Aminobutyric acid
gamma-Aminobutyric acid
03 medical and health sciences
Pollen
Tobacco
otorhinolaryngologic diseases
medicine
Extracellular
γ-Aminobutyric acid
gamma-Aminobutyric Acid
030304 developmental biology
Plant Proteins
0303 health sciences
Voltage-dependent calcium channel
biology
Nicotiana tabacum
Glutamate Decarboxylase
Cell Membrane
food and beverages
Cell biology
cell–cell communication
Biochemistry
biology.protein
Pollen tube
Calcium Channels
pollen tube
010606 plant biology & botany
medicine.drug
Research Paper
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 14602431 and 00220957
- Volume :
- 65
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....e8dd846621ac65448b39c03c17e02758