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Evaluation of Caripill™ as a component of platelet storage solution
- Source :
- Hematology, Transfusion and Cell Therapy, Vol 43, Iss 2, Pp 133-140 (2021), Hematology, Transfusion and Cell Therapy, Hematology, Transfusion and Cell Therapy, Volume: 43, Issue: 2, Pages: 133-140, Published: 07 JUL 2021
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Background Carica papaya Linn. has high nutraceutical and pharmacological values. The leaves possess antimicrobial, anti-tumor and antioxidant properties. They are used to treat thrombocytopenia during dengue fever and the leaf extract is commercially available as tablets under the name Caripill™ (MicroLabs, Bengaluru). Nevertheless, platelet transfusion is recommended in severe cases of thrombocytopenia, but the platelet storage is limited to 5–7 days at 22−24 °C. Reducing oxidative stress (OS) during platelet storage might help in prolonging the shelf-life, since the OS is known to cause platelet storage lesion. Hence, this study investigated the effects of Caripill™ as an additive in Tyrode’s buffer during extended platelet storage. Methods Platelets isolated from 4 months old male Wistar rats were stored with Caripill™ (50 and 100 μg/ml) at 22 °C for 12 days. Platelet functional and metabolic markers and various OS markers were analyzed on days 0, 4, 8 and 12. Results Caripill™ (50 and 100 μg/ml) maintained platelet functions and lactate dehydrogenase, elevated nitrites, reduced glucose consumption, protected proteins and up-regulated the antioxidant enzymes. However, the CP100 up-regulated catalase from day 4, elevated nitrites from day 8, prevented the formation of secondary products of lipid peroxidation and increased the total antioxidant capacity on day 4. Conclusions Caripill™ reduced platelet storage lesion up to day 8 of storage. Results suggest that a higher concentration of Caripill™ was more effective in combating the oxidative damage during platelet storage. This study throws light on the beneficial effects of Caripill™ in combating oxidative stress during platelet storage.
- Subjects :
- Platelets
Antioxidant
medicine.medical_treatment
Storage
030204 cardiovascular system & hematology
Pharmacology
medicine.disease_cause
Lesion
Lipid peroxidation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Lactate dehydrogenase
Immunology and Allergy
Medicine
Platelet
Diseases of the blood and blood-forming organs
biology
business.industry
Carica papaya
Hematology
Platelet transfusion
chemistry
Catalase
Oxidative stress
Caripill™
biology.protein
Original Article
medicine.symptom
RC633-647.5
business
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 25311379
- Volume :
- 43
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Hematology, Transfusion and Cell Therapy
- Accession number :
- edsair.doi.dedup.....e8ae77d824f97733c7af78db783ad65e