Back to Search
Start Over
Investigating the Cellular Transcriptomic Response Induced by the Makona Variant of Ebola Virus in Differentiated THP-1 Cells
- Source :
- Viruses, Volume 11, Issue 11, Viruses, Vol 11, Iss 11, p 1023 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Recent studies have shown that transcriptomic analysis of blood samples taken from patients with acute Ebola virus disease (EVD) during the 2013&ndash<br />2016 West African outbreak was suggestive that a severe inflammatory response took place in acutely ill patients. The significant knowledge gained from studying the Makona variant, a cause of the largest known EVD outbreak, may be applicable to other species of ebolavirus, and other variants of the Ebola virus (EBOV) species. To investigate the ability of Makona to initiate an inflammatory response in human macrophages and characterise the host response in a similar manner to previously characterised EBOV variants, the human monocytic cell line THP-1 was differentiated into macrophage-like cells and infected with Makona. RNA-Seq and quantitative proteomics were used to identify and quantify host mRNA and protein abundance during infection. Data from infection with Reston virus (RESTV) were used as comparators to investigate changes that may be specific to, or enhanced in, Makona infection in relation to a less pathogenic species of ebolavirus.. This study found demonstrable induction of the inflammatory response, and increase in the activation state of THP-1 macrophages infected with Makona. NF&kappa<br />B and inflammation-associated transcripts displayed significant changes in abundance, reflective of what was observed in human patients during the 2013&ndash<br />2016 EBOV outbreak in West Africa, and demonstrated that transcriptomic changes found in Makona-infected cells were similar to that observed in Reston virus infection and that have been described in previous studies of other variants of EBOV.
- Subjects :
- 0301 basic medicine
THP-1 Cells
viruses
030106 microbiology
lcsh:QR1-502
Disease
Biology
medicine.disease_cause
Virus
Article
lcsh:Microbiology
Transcriptome
03 medical and health sciences
Ebola virus
Makona
transcriptomics
proteomics
Virology
West Africa
medicine
Humans
THP1 cell line
Ebolavirus
Gene Expression Profiling
Macrophages
Outbreak
virus diseases
Hemorrhagic Fever, Ebola
030104 developmental biology
Infectious Diseases
Gene Expression Regulation
Cell culture
Cytokines
Interferons
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....e8415e683b0dc8f654a17f2ba4f06458
- Full Text :
- https://doi.org/10.3390/v11111023