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Thyroid Hormone Deficiency Modifies Hepatic Lipid Droplet Morphology and Molecular Properties in Lithogenic-Diet Supplemented Mice
- Source :
- Experimental and Clinical Endocrinology & Diabetes. 129:926-930
- Publication Year :
- 2021
- Publisher :
- Georg Thieme Verlag KG, 2021.
-
Abstract
- Objective Thyroid hormones have been associated with a hepatic lipid lowering effect and thyroid function has been shown to play a substantial role in development of non-alcoholic fatty liver disease. Hepatic lipid droplets differ in the number, size and molecular properties depending on metabolic state or pathological condition. However, in how far thyroid hormone deficiency affects hepatic lipid droplet morphology and molecular properties is still poorly understood. Therefore, we performed a study in mice using a lithogenic diet model of steatohepatitis and modulated the thyroid hormone status. Methods Male and female three months old C57BL/6 mice were divided into a euthyroid (control), a lithogenic (litho) and a lithogenic+thyroid hormone deficient (litho+hypo) group and treated for six weeks. Hepatic transmission electron microscopy and gene expression analysis of lipid-droplet associated proteins were performed. Results Increased mean diameters of hepatic lipid droplets and a shift towards raised electron-density in lipid droplets was observed under thyroid hormone deficiency. Furthermore thyroid hormone deficiency altered hepatic expression of genes involved in lipophagy and triacylglycerol mobilization. Interestingly, while the impact of thyroid hormone deficiency on lipid droplet morphology seems to be sex-independent, hepatic lipid droplet-associated gene expression differed significantly between both sexes. Conclusion This study demonstrates that thyroid hormone deficiency alters hepatic lipid droplet morphology and hepatic gene expression of lipid droplet-associated proteins in a lithogenic diet mouse model of steatohepatitis.
- Subjects :
- 0301 basic medicine
Thyroid Hormones
endocrine system
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Medizin
Mice
03 medical and health sciences
0302 clinical medicine
Endocrinology
Hypothyroidism
Cholelithiasis
Lipid droplet
Internal medicine
Gene expression
Internal Medicine
medicine
Animals
Euthyroid
Chemistry
Liver Diseases
Fatty liver
Thyroid
Lipid Droplets
General Medicine
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Hepatocytes
Female
Steatohepatitis
Thyroid function
Hormone
Subjects
Details
- ISSN :
- 14393646 and 09477349
- Volume :
- 129
- Database :
- OpenAIRE
- Journal :
- Experimental and Clinical Endocrinology & Diabetes
- Accession number :
- edsair.doi.dedup.....e840db0ecbef0bee33f29f48246b291c