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Reducing the content of nornicotine in tobacco via targeted mutation breeding
- Source :
- Molecular Breeding, Molecular Breeding, Springer Verlag, 2008, 21 (3), pp.369-381. ⟨10.1007/s11032-007-9138-2⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- International audience; Cultivated tobacco produces secondary alkaloids involved in the formation of nitrosamines with health concerns. The recent identification of target genes in nicotine and nornicotine biosynthetic pathways now allows biotechnological approaches for their control. We demonstrate here that mutation breeding can be used as an alternative to genetically modified (GM) plants for generating nornicotine-free tobacco. Ten alleles of the NtabCYP82E4 gene (nicotine N-demethylase) were identified by screening 1,311 M2 families of tobacco ethylmethane sulphonate (EMS) mutants. Alkaloid analysis indicated that the nornicotine contents of homozygous M2 plants carrying nonsense or missense alleles of NtabCYP82E4 were very low or near-null. Backcrossing with tobacco elite varieties yielded BC1 plants phenotypically undistinguishable from parental lines. This major objective of tobacco breeders in the last few decades could be reached in a period of less than 1.5 years, including the creation of highly mutagenised tobacco mutant collections and the detection of mutated alleles using a simple and versatile detection technology (capillary electrophoresis-single strand conformation polymorphism, CE-SSCP) accessible to most breeding companies and crop species.
- Subjects :
- 0106 biological sciences
Nornicotine
Nicotiana tabacum
[SDV]Life Sciences [q-bio]
Mutagenesis (molecular biology technique)
NORNICOTINE
Plant Science
01 natural sciences
Nicotine
03 medical and health sciences
chemistry.chemical_compound
Genetics
medicine
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
CE-SSCP
Molecular Biology
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
2. Zero hunger
0303 health sciences
BIOLOGIE VEGETALE
Mutation breeding
biology
business.industry
biology.organism_classification
Biotechnology
Genetically modified organism
Targeted Mutation
chemistry
Backcrossing
business
Agronomy and Crop Science
010606 plant biology & botany
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 13803743 and 15729788
- Database :
- OpenAIRE
- Journal :
- Molecular Breeding, Molecular Breeding, Springer Verlag, 2008, 21 (3), pp.369-381. ⟨10.1007/s11032-007-9138-2⟩
- Accession number :
- edsair.doi.dedup.....e7a36ea95515a137f9858bb1b656cefa