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Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity

Authors :
Vanessa Demonfort Nkamga
Ahmed Loukil
Elisabeth Sogodogo
Mustapha Fellag
Michel Drancourt
Patrick Dessi
Justin Michel
Pierre-Edouard Fournier
Microbes évolution phylogénie et infections (MEPHI)
Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Vecteurs - Infections tropicales et méditerranéennes (VITROME)
Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA)
Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)
Source :
Frontiers in Public Health, Frontiers in Public Health, Frontiers Media S.A., 2019, 7, ⟨10.3389/fpubh.2019.00038⟩, Frontiers in Public Health, Vol 7 (2019), Frontiers in Public Health, 2019, 7, ⟨10.3389/fpubh.2019.00038⟩
Publication Year :
2019
Publisher :
Frontiers Media S.A., 2019.

Abstract

The authors report the cases of 9 patients eventually diagnosed with methanogenic archaea refractory or recalcitrant chronic rhinosinusitis, a condition known to involve various anaerobic bacteria but in which the role of methanogenic archaea is unknown. The authors retrospectively searched these microorganisms by PCR in surgically-collected sinusal pus specimens from patients diagnosed with refractory sinusitis, defined by the persistance of sinus inflammation and related-symptoms for more than 12 weeks despite appropriate treatment. Of the 116 tested sinus surgical specimens, 12 (10.3%) from 9 patients (six females, three males; aged 20–71 years) were PCR-positive. These specimens were further investigated by fluorescence in-situ hybridization, PCR amplicon-sequencing and culture. Methanobrevibacter smithii was documented in four patients and Methanobrevibacter oralis in another four, one of whom was also culture-positive. They were associated with a mixed flora including Gram-positive and Gram-negative bacteria. In the latter patient, “Methanobrevibacter massiliense” was the sole microorganism detected. These results highlight methanogenic archaea as being part of a mixed anaerobic flora involved in refractory sinusitis, and suggest that the treatment of this condition should include an antibiotic active against methanogens, notably a nitroimidazole derivative.

Details

Language :
English
ISSN :
22962565
Volume :
7
Database :
OpenAIRE
Journal :
Frontiers in Public Health
Accession number :
edsair.doi.dedup.....e79995fa2bf57b4d228a261eab2450dd