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Functional analysis of alternative castor bean DGAT enzymes

Authors :
Thomaz Stumpf Trenz
Andreia Carina Turchetto-Zolet
Rogério Margis
Marcia Margis-Pinheiro
Felipe dos Santos Maraschin
Source :
Genetics and Molecular Biology v.46 n.1 suppl.1 2023, Genetics and Molecular Biology, Sociedade Brasileira de Genética (SBG), instacron:SBG, Genetics and Molecular Biology, Volume: 46, Issue: 1 Supplement 1, Article number: e20220097, Published: 09 DEC 2022
Publication Year :
2023
Publisher :
Sociedade Brasileira de Genética, 2023.

Abstract

The diversity of diacylglycerol acyltransferases (DGATs) indicates alternative roles for these enzymes in plant metabolism besides triacylglycerol (TAG) biosynthesis. In this work, we functionally characterized castor bean (Ricinus communis L.) DGATs assessing their subcellular localization, expression in seeds, capacity to restore triacylglycerol (TAG) biosynthesis in mutant yeast and evaluating whether they provide tolerance over free fatty acids (FFA) in sensitive yeast. RcDGAT3 displayed a distinct subcellular localization, located in vesicles outside the endoplasmic reticulum (ER) in most leaf epidermal cells. This enzyme was unable to restore TAG biosynthesis in mutant yeast; however, it was able to outperform other DGATs providing higher tolerance over FFA. RcDAcTA subcellular localization was associated with the ER membranes, resembling RcDGAT1 and RcDGAT2, but it failed to rescue the long-chain TAG biosynthesis in mutant yeast, even with fatty acid supplementation. Besides TAG biosynthesis, our results suggest that RcDGAT3 might have alternative functions and roles in lipid metabolism.

Details

Language :
English
Database :
OpenAIRE
Journal :
Genetics and Molecular Biology v.46 n.1 suppl.1 2023, Genetics and Molecular Biology, Sociedade Brasileira de Genética (SBG), instacron:SBG, Genetics and Molecular Biology, Volume: 46, Issue: 1 Supplement 1, Article number: e20220097, Published: 09 DEC 2022
Accession number :
edsair.doi.dedup.....e78a5e893ffeed7df7fe7610cae512cf