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The response of the root proteome to the synthetic strigolactone GR24 in Arabidopsis
- Source :
- Molecular and Cellular Proteomics, Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2016, 15 (8), pp.2744-2755. ⟨10.1074/mcp.M115.050062⟩, MOLECULAR & CELLULAR PROTEOMICS
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; Strigolactones are plant metabolites that act as phytohormones and rhizosphere signals. Whereas most research on unraveling the action mechanisms of strigolactones is focused on plant shoots, we investigated proteome adaptation during strigolactone signaling in the roots of Arabidopsis thaliana. Through large-scale, time-resolved, and quantitative proteomics, the impact of the strigolactone analog rac-GR24 was elucidated on the root proteome of the wild type and the signaling mutant more axillary growth 2 (max2). Our study revealed a clear MAX2-dependent rac-GR24 response: an increase in abundance of enzymes involved in flavonol biosynthesis, which was reduced in the max2-1 mutant. Mass spectrometry-driven metabolite profiling and thin-layer chromatography experiments demonstrated that these changes in protein expression lead to the accumulation of specific flavonols. Moreover, quantitative RT-PCR revealed that the flavonol-related protein expression profile was caused by rac-GR24-induced changes in transcript levels of the corresponding genes. This induction of flavonol production was shown to be activated by the two pure enantiomers that together make up rac-GR24. Finally, our data provide much needed clues concerning the multiple roles played by MAX2 in the roots and a comprehensive view of the rac-GR24-induced response in the root proteome.
- Subjects :
- Proteomics
0106 biological sciences
0301 basic medicine
Flavonols
[SDV]Life Sciences [q-bio]
Arabidopsis
ACTS DOWNSTREAM
Plant Roots
01 natural sciences
Biochemistry
Mass Spectrometry
ANALOG GR24
Analytical Chemistry
Lactones
Gene Expression Regulation, Plant
[SDV.IDA]Life Sciences [q-bio]/Food engineering
PHOSPHATE
Arabidopsis thaliana
Rhizosphere
biology
Proteome
[SDE]Environmental Sciences
SHOOT
Heterocyclic Compounds, 3-Ring
Plant Shoots
MAX1
GENES
PROTEINS
Quantitative proteomics
Strigolactone
03 medical and health sciences
HORMONE
Metabolomics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Molecular Biology
Arabidopsis Proteins
Research
FLAVONOLS
Biology and Life Sciences
biology.organism_classification
030104 developmental biology
Mutation
Carrier Proteins
AUXIN TRANSPORT
Chromatography, Liquid
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 15359476 and 15359484
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Proteomics, Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2016, 15 (8), pp.2744-2755. ⟨10.1074/mcp.M115.050062⟩, MOLECULAR & CELLULAR PROTEOMICS
- Accession number :
- edsair.doi.dedup.....e7881b0fb5e9a67c4305ed07129ab508