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Deciphering the microRNA transcriptome of skeletal muscle during porcine development
- Source :
- PeerJ, Vol 4, p e1504 (2016), PeerJ
- Publication Year :
- 2016
- Publisher :
- PeerJ, 2016.
-
Abstract
- MicroRNAs (miRNAs) play critical roles in many important biological processes, such as growth and development in mammals. Various studies of porcine muscle development have mainly focused on identifying miRNAs that are important for fetal and adult muscle development; however, little is known about the role of miRNAs in middle-aged muscle development. Here, we present a comprehensive investigation of miRNA transcriptomes across five porcine muscle development stages, including one prenatal and four postnatal stages. We identified 404 known porcine miRNAs, 118 novel miRNAs, and 101 miRNAs that are conserved in other mammals. A set of universally abundant miRNAs was found across the distinct muscle development stages. This set of miRNAs may play important housekeeping roles that are involved in myogenesis. A short time-series expression miner analysis indicated significant variations in miRNA expression across distinct muscle development stages. We also found enhanced differentiation- and morphogenesis-related miRNA levels in the embryonic stage; conversely, apoptosis-related miRNA levels increased relatively later in muscle development. These results provide integral insight into miRNA function throughout pig muscle development stages. Our findings will promote further development of the pig as a model organism for human age-related muscle disease research.
- Subjects :
- 0301 basic medicine
Porcine
Middle-aged
ved/biology.organism_classification_rank.species
lcsh:Medicine
Biology
Bioinformatics
General Biochemistry, Genetics and Molecular Biology
Transcriptome
03 medical and health sciences
0302 clinical medicine
microRNA
Genetics
medicine
Prenatal
Muscle development
Agricultural Science
Model organism
Molecular Biology
miRNA
Fetus
ved/biology
Myogenesis
General Neuroscience
lcsh:R
Skeletal muscle
Embryonic Stage
Genomics
General Medicine
Cell biology
Postnatal
030104 developmental biology
medicine.anatomical_structure
General Agricultural and Biological Sciences
030217 neurology & neurosurgery
Function (biology)
Subjects
Details
- ISSN :
- 21678359
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....e77538ab6156e4e0ccd9edbf581da60b