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Mouse lymphomyeloid cells can function with significantly decreased expression levels of cytochrome C

Authors :
Sergei A. Nedospasov
Marina S. Drutskaya
R. V. Zvartsev
Vladimir P. Skulachev
I. V. Kislyakov
E. A. Gorshkova
E. S. Shilov
Ilgiz A. Mufazalov
Source :
Biochemistry. Biokhimiia. 79(12)
Publication Year :
2015

Abstract

Cytochrome c is an indispensable electron carrier in the mitochondrial respiratory chain and also an important mediator of the internal pathway triggering apoptosis. Mice with a complete deficiency of the Cycs gene encoding the somatic cytochrome c die during the embryogenesis. Using the technology of LoxP-cre-dependent tissue-specific recombination, we obtained some mouse strains with significantly reduced expression of cytochrome c in certain cell types ("conditional genetic knockdown"). This knockdown was achieved by abrogation of the normal splicing of the Cycs locus pre-mRNA due to an additional acceptor site inside the stop-cassette neo(r). Previously, we observed embryonic lethality in homozygous mice with the same knockdown of cytochrome c in all cells of the organism. In the present work we studied two novel mouse strains with conditional knockdown of the Cycs gene in T lymphocytes and macrophages. Somewhat surprisingly, the mice of these two strains under normal conditions were not phenotypically different from the wild-type mice, either on the whole organism level or on the level of activity of individual target cells. Thus, the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning.

Details

ISSN :
16083040
Volume :
79
Issue :
12
Database :
OpenAIRE
Journal :
Biochemistry. Biokhimiia
Accession number :
edsair.doi.dedup.....e773b0bfc3f3d20ab14f0f92bf689b43