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LOCAL APPLICATION OF NUCLEUS PULPOSUS INDUCES EXPRESSION OF P2X3 IN RAT DORSAL ROOT GANGLION CELLS

Authors :
Björn Rydevik
Kaoru Takahashi Sato
Masahiro Murakawa
Kjell Olmarker
Shinichi Kikuchi
Koichiro Satoh
Shinichi Konno
Miho Sekiguchi
Source :
FUKUSHIMA JOURNAL OF MEDICAL SCIENCE. 58:17-21
Publication Year :
2012
Publisher :
The Fukushima Society of Medical Science, 2012.

Abstract

The P2X(3) receptor is a ligand-gated cation channel that is activated by extra cellular adenosine triphosphate (ATP) found in the dorsal root, trigeminal and nodose ganglia. It is one of the receptors transmitting nociceptive information of injuries and inflammation of the periphery by endogenous ATP released from damaged cells. The present study was performed in order to evaluate if there was an increased expression of P2X(3)-immunoreactivity in dorsal root ganglion (DRG) neurons after experimental disc herniation. There were four groups: exposure of the left L4 dorsal root ganglion and incision of the L4-L5 disc, exposure and slight displacement of the left L4 dorsal ganglion, sham exposure of the L4 dorsal root ganglion, and normal. Seven days after surgery, the DRG's were collected, sectioned and stained immunohistochemically for the P2X(3) receptor. The expression of P2X(3) increased significantly following incision of the L4-5 disc compared to the normal group. Sham surgery induced a minor, although statistically significant increase. Mechanical displacement did not induce any increased expression of the receptors. The study demonstrates that expression of the P2X(3)receptors in the DRG may be induced by local application of nucleus pulposus. This may increase our understanding of the pathophysiologic mechanisms related to disc herniation and sciatica.

Details

ISSN :
21854610 and 00162590
Volume :
58
Database :
OpenAIRE
Journal :
FUKUSHIMA JOURNAL OF MEDICAL SCIENCE
Accession number :
edsair.doi.dedup.....e75e092247173969dc9163bf9dd1350d
Full Text :
https://doi.org/10.5387/fms.58.17