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Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- Genetic modification of the flavonoid pathway has been used to produce novel colours and colour patterns in ornamental plants as well as to modify the nutritional and pharmaceutical properties of food crops. It has been suggested that co-ordinate control of multiple steps of the pathway with the help of regulatory genes would lead to a more predictable control of metabolic flux. Regulation of anthocyanin biosynthesis has been studied in a common ornamental plant, Gerbera hybrida (Asteraceae). An R2R3-type MYB factor, GMYB10, shares high sequence similarity and is phylogenetically grouped together with previously characterized regulators of anthocyanin pigmentation. Ectopic expression of GMYB10 leads to strongly enhanced accumulation of anthocyanin pigments as well as to an altered pigmentation pattern in transgenic gerbera plants. Anthocyanin analysis indicates that GMYB10 specifically induces cyanidin biosynthesis in undifferentiated callus and in vegetative tissues. Furthermore, in floral tissues enhanced pelargonidin production is detected. Microarray analysis using the gerbera 9K cDNA array revealed a highly predicted set of putative target genes for GMYB10 including new gene family members of both early and late biosynthetic genes of the flavonoid pathway. However, completely new candidate targets, such as a serine carboxypeptidase-like gene as well, as two new MYB domain factors, GMYB11 and GMYB12, whose exact function in phenylpropanoid biosynthesis is not clear yet, were also identified.
- Subjects :
- Anthocyanin
0106 biological sciences
Gerbera
Physiology
Molecular Sequence Data
MYB
Flowers
Plant Science
Asteraceae
01 natural sciences
Anthocyanins
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Genes, Regulator
genetic modification
Gene family
flavonoid
Gene
Plant Proteins
030304 developmental biology
Regulator gene
Genetics
0303 health sciences
Phenylpropanoid
biology
fungi
food and beverages
biology.organism_classification
Research Papers
Phenotype
chemistry
Ectopic expression
Transcription Factors
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....e73f32bff0a18d28a82487d490d68465