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Biomolecular engineering of biocatalysts hydrolyzing neurotoxic organophosphates
- Source :
- Biochimie. 144
- Publication Year :
- 2017
-
Abstract
- Novel methods of molecular modeling help solving urgent problems in drug design, directed evolution of biocatalysts and biosensors, and a lot of other research fields. Implementation of such methods to organophosphorus hydrolase being perfect research object that hydrolyzes dangerous neurotoxic organophosphates could intensify development of antidote and protective preparations to treat poisoning. Structures of enzyme-polyelectrolyte complexes (EPCs) based on hexahistidine-tagged organophosphorus hydrolase (His6-OPH) with different biopolymers (various modifications of polyglutamic and polyaspartic acid, as well as hydroxyethyl starch and succinylated gelatin) were simulated at different pH using molecular docking. A number of EPCs with expected “positive” effect on maintaining the maximum level of His6-OPH activity, and some “negative” options were produced, and their catalytic performance was studied. The theoretical results were experimentally confirmed for four of the six “positive” options. EPCs obtained possessed up to 20–40% higher catalytic efficiency in hydrolysis reactions of Paraoxon and Parathion-methyl as compared with that of the native His6-OPH. The results obtained may be a good proof of concept for implementation of molecular docking to calculate model complexes of proteins with (bio)polymers of 6.4–105.5 kg/mol. Also, the approach used here could be interesting as alternative or addition to the directed modifications of enzymes to alter their catalytic characteristics.
- Subjects :
- 0301 basic medicine
Molecular model
Hydrolases
Protein Conformation
Chemical structure
Neurotoxins
Biomolecular engineering
Bioengineering
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Hydrolase
Organophosphorus compound
medicine
Organic chemistry
chemistry.chemical_classification
Paraoxon
Hydrolysis
General Medicine
Directed evolution
Organophosphates
Molecular Docking Simulation
030104 developmental biology
chemistry
Biocatalysis
Polyaspartic acid
medicine.drug
Subjects
Details
- ISSN :
- 16386183
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....e731e36b34c9231c0504f68406835e1b